Details der Publikation - Generation and immunogenicity of virus-like particles based on mink enteritis virus capsid protein VP2 expressed in Sf9 cells

Generation and immunogenicity of virus-like particles based on mink enteritis virus capsid protein VP2 expressed in Sf9 cells

Abstract Mink enteritis virus (MEV) is a parvovirus that causes acute enteritis in mink. The capsid protein VP2 of MEV is a major immunogenicity that is important for disease prevention. In this study, this protein was expressed in Spodoptera frugiperda 9 cells using a recombinant baculovirus system and was observed to self-assemble into virus-like particles (VLPs) with a high hemagglutination (HA) titer (1:$ 2^{16} $). A single-dose injection of VLPs (HA titer, 1:256) resulted in complete protection of mink against virulent MEV challenge for at least 180 days. These data suggest that these MEV VLPs could be used as a vaccine for the prevention of viral enteritis in mink..

Medienart:	E-Artikel

Erscheinungsjahr:	2020
Erschienen:	2020

Enthalten in:	Zur Gesamtaufnahme - volume:165
Enthalten in:	Archives of virology - 165(2020), 9 vom: 01. Juli, Seite 2065-2071

Sprache:	Englisch

Beteiligte Personen:	Wu, Hongchao [VerfasserIn] Jin, Hongli [VerfasserIn] Wang, Lingxiao [VerfasserIn] Huo, Ningning [VerfasserIn] Liu, Di [VerfasserIn] Ding, Hangtian [VerfasserIn] Cao, Yujiao [VerfasserIn] Liu, Caihong [VerfasserIn] Xi, Xiangfeng [VerfasserIn] Jiao, Cuicui [VerfasserIn] Spibey, Norman [VerfasserIn] Shi, Jing [VerfasserIn] Liu, Yuxiu [VerfasserIn] Tian, Kegong [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

RVK:	RVK Klassifikation ELIB24 ELIB09

Anmerkungen:	© Springer-Verlag GmbH Austria, part of Springer Nature 2020

doi:	10.1007/s00705-020-04703-6

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2118741375

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520			\|a Abstract Mink enteritis virus (MEV) is a parvovirus that causes acute enteritis in mink. The capsid protein VP2 of MEV is a major immunogenicity that is important for disease prevention. In this study, this protein was expressed in Spodoptera frugiperda 9 cells using a recombinant baculovirus system and was observed to self-assemble into virus-like particles (VLPs) with a high hemagglutination (HA) titer (1:$ 2^{16} $). A single-dose injection of VLPs (HA titer, 1:256) resulted in complete protection of mink against virulent MEV challenge for at least 180 days. These data suggest that these MEV VLPs could be used as a vaccine for the prevention of viral enteritis in mink.
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912			\|a GBV_ILN_2112
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912			\|a GBV_ILN_2118
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Generation and immunogenicity of virus-like particles based on mink enteritis virus capsid protein VP2 expressed in Sf9 cells

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