Details der Publikation - Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize

Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize

Abstract Insect-associated microbes can contribute to the physiological and ecological functions of insects. Despite a few examples in beetles and piercing-sucking insects, the varied mechanisms of how insect-associated bacteria mediate plant-insect interactions are still not fully understood. The polyphagous herbivore Helicoverpa zea is a major agricultural pest that harbors certain microbes in their digestive systems. Enterobacter ludwigii is one of the gut-associated bacteria identified from field-collected caterpillars, and it has been shown to indirectly induce defenses in the dicot plant tomato by triggering the biosynthesis of salivary elicitors, but there are no clear mechanisms to show how gut microbes alter these salivary cues and how a different host plant responds to these inducible elicitors. Here, we conducted a series of assays to determine whether infection with E. ludwigii affects H. zea larval growth, immunity, and salivary responses and thus influences induced defenses of maize to herbivory. Inoculating lab-reared caterpillars with E. ludwigii, did not significantly affect the growth of caterpillars, but two immunity-related genes glucose oxidase (GOX) and lysozyme (LYZ) were more highly expressed in both salivary glands and midguts compared with $ MgCl_{2} $ solution-treated caterpillars. Oral elicitors were evaluated for their role in triggering maize-specific defense responses. Our results show that saliva and its main component protein glucose oxidase (GOX) from E. ludwigii-inoculated caterpillars played a role in inducing maize anti-herbivore responses. These findings provide a novel concept that introducing bacteria to an herbivore may be an important approach to pest control through alteration of insect immune responses and thus indirect induction of plant resistance..

Medienart:	E-Artikel

Erscheinungsjahr:	2018
Erschienen:	2018

Enthalten in:	Zur Gesamtaufnahme - volume:44
Enthalten in:	Journal of chemical ecology - 44(2018), 7-8 vom: 22. Mai, Seite 690-699

Sprache:	Englisch

Beteiligte Personen:	Wang, Jie [VerfasserIn] Yang, Mingyu [VerfasserIn] Song, Yuanyuan [VerfasserIn] Acevedo, Flor E. [VerfasserIn] Hoover, Kelli [VerfasserIn] Zeng, Rensen [VerfasserIn] Felton, Gary W. [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	43.12$jUmweltchemie
Themen:	Glucose oxidase Gut bacteria Immunity Induced defense Microbiome Saliva Symbiosis

Anmerkungen:	© Springer Science+Business Media, LLC, part of Springer Nature 2018. corrected publication June/2018

doi:	10.1007/s10886-018-0970-0

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2115562623

Internformat


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520			\|a Abstract Insect-associated microbes can contribute to the physiological and ecological functions of insects. Despite a few examples in beetles and piercing-sucking insects, the varied mechanisms of how insect-associated bacteria mediate plant-insect interactions are still not fully understood. The polyphagous herbivore Helicoverpa zea is a major agricultural pest that harbors certain microbes in their digestive systems. Enterobacter ludwigii is one of the gut-associated bacteria identified from field-collected caterpillars, and it has been shown to indirectly induce defenses in the dicot plant tomato by triggering the biosynthesis of salivary elicitors, but there are no clear mechanisms to show how gut microbes alter these salivary cues and how a different host plant responds to these inducible elicitors. Here, we conducted a series of assays to determine whether infection with E. ludwigii affects H. zea larval growth, immunity, and salivary responses and thus influences induced defenses of maize to herbivory. Inoculating lab-reared caterpillars with E. ludwigii, did not significantly affect the growth of caterpillars, but two immunity-related genes glucose oxidase (GOX) and lysozyme (LYZ) were more highly expressed in both salivary glands and midguts compared with $ MgCl_{2} $ solution-treated caterpillars. Oral elicitors were evaluated for their role in triggering maize-specific defense responses. Our results show that saliva and its main component protein glucose oxidase (GOX) from E. ludwigii-inoculated caterpillars played a role in inducing maize anti-herbivore responses. These findings provide a novel concept that introducing bacteria to an herbivore may be an important approach to pest control through alteration of insect immune responses and thus indirect induction of plant resistance.
650		4	\|a Gut bacteria
650		4	\|a Induced defense
650		4	\|a Symbiosis
650		4	\|a Microbiome
650		4	\|a Immunity
650		4	\|a Saliva
650		4	\|a Glucose oxidase
700	1		\|a Yang, Mingyu \|4 aut
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700	1		\|a Acevedo, Flor E. \|4 aut
700	1		\|a Hoover, Kelli \|4 aut
700	1		\|a Zeng, Rensen \|4 aut
700	1		\|a Felton, Gary W. \|4 aut
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Gut-Associated Bacteria of Helicoverpa zea Indirectly Trigger Plant Defenses in Maize

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