The effect of short-term exposure of cumulus-oocyte complexes to in vitro maturation medium on yield of mature oocytes and usable embryos in stimulated cycles

Purpose We examined whether short-term exposure to in vitro maturation (IVM) medium of cumulus-oocyte complexes (COCs) from a stimulated cycle increases the yield of metaphase II (MII) oocytes and usable embryos. Methods Retrospective review of two consecutive autologous IVF/ICSI cycles per patient between 2007 and 2015 in which cycle 1 did not result in live birth. Patients with short-term exposure of COCs to IVM medium (3–5 h before standard insemination or ICSI) in cycle 2 (treated) were matched 1:4 on %MI and %MII to patients without use of IVM in cycle 2 (untreated). The proportions of mature oocytes, two pronucleate (2PN) zygotes, number of usable embryos, and clinical outcomes were compared between groups with regression modeling. Results The treated (n = 43) and untreated (n = 163) groups had similar demographic characteristics and similarly high proportions of immature oocytes (48.2 vs. 41.3%, respectively) in cycle 1. There were no significant differences between the treated and untreated groups in the change in %MII (48.1 to 68.9% vs. 50.5 to 72.5%, respectively) or mean number of usable embryos (2.2 to 3.4 vs. 2.0 to 3.3, respectively) from cycle 1 to cycle 2. Conclusions These findings suggest that short-term IVM incubation of COCs may not provide any additional benefit in patients with a prior unsuccessful cycle notable for a high proportion of immature oocytes. Further randomized studies are warranted to determine whether there is a subset of patients who may have improved clinical outcomes with this “rescue IVM” intervention..

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:35

Enthalten in:

Journal of assisted reproduction and genetics - 35(2018), 5 vom: 14. März, Seite 841-849

Sprache:

Englisch

Beteiligte Personen:

Sacha, C. R. [VerfasserIn]
Kaser, D. J. [VerfasserIn]
Farland, L. V. [VerfasserIn]
Srouji, S. [VerfasserIn]
Missmer, S. A. [VerfasserIn]
Racowsky, C. [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

BKL:

44.92$jGynäkologie

Themen:

IVF
In vitro maturation
Maturity
Oocytes

Anmerkungen:

© Springer Science+Business Media, LLC, part of Springer Nature 2018

doi:

10.1007/s10815-018-1155-z

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2114729990

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