Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium
Purpose In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C. Methods Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min. Results Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21. Conclusion This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter..
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2009 |
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Erschienen: |
2009 |
Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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Enthalten in: |
Journal of assisted reproduction and genetics - 26(2009), 8 vom: Aug., Seite 467-473 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Datta, Uttam [VerfasserIn] |
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Links: |
Volltext [lizenzpflichtig] |
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BKL: | |
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Themen: |
Coenzyme Q10 |
Anmerkungen: |
© Springer Science+Business Media, LLC 2009 |
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doi: |
10.1007/s10815-009-9344-4 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
OLC2114711676 |
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100 | 1 | |a Datta, Uttam |e verfasserin |4 aut | |
245 | 1 | 0 | |a Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium |
264 | 1 | |c 2009 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
500 | |a © Springer Science+Business Media, LLC 2009 | ||
520 | |a Purpose In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C. Methods Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min. Results Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21. Conclusion This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter. | ||
650 | 4 | |a Coenzyme Q10 | |
650 | 4 | |a Electrolyte free medium | |
650 | 4 | |a Epididymal spermatozoa | |
650 | 4 | |a Freezing | |
650 | 4 | |a Hypo-osmotic swelling test | |
700 | 1 | |a Chandra Sekar, M. |4 aut | |
700 | 1 | |a Hembram, Manik Lal |4 aut | |
700 | 1 | |a Dasgupta, Raju |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of assisted reproduction and genetics |d Springer US, 1984 |g 26(2009), 8 vom: Aug., Seite 467-473 |h Online-Ressource |w (DE-627)320573060 |w (DE-600)2016722-2 |w (DE-576)121191648 |x 1573-7330 |7 nnns |
773 | 1 | 8 | |g volume:26 |g year:2009 |g number:8 |g month:08 |g pages:467-473 |
856 | 4 | 0 | |u https://dx.doi.org/10.1007/s10815-009-9344-4 |z lizenzpflichtig |3 Volltext |
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