Quantitative and qualitative extraction of RNA from a filamentous cyanobacterium Anabaena variabilis PCC 7937
Abstract Cyanobacteria are rich in polysaccharides and also have a multilayered cell wall that interferes with the successful extraction of RNA. The methods available to extract RNA from cyanobacteria usually give low yield and poor quality of RNA having DNA as contaminant that requires additional treatment with DNase before performing any gene expression studies. Here, we present a simple and rapid method for the extraction of RNA from a filamentous cyanobacterium, Anabaena variabilis PCC 7937, that gives high yield and good quality of RNA without DNA contamination as tested by spectrophotometry, agarose gel electrophoresis, and reverse transcriptase polymerase chain reaction analysis..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Journal of applied phycology - 22(2009), 1 vom: 19. März, Seite 113-116 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Singh, Shailendra P. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Agarose gel electrophoresis |
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| Anmerkungen: |
© Springer Science+Business Media B.V. 2009 |
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| doi: |
10.1007/s10811-009-9428-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2114659364 |
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| 100 | 1 | |a Singh, Shailendra P. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quantitative and qualitative extraction of RNA from a filamentous cyanobacterium Anabaena variabilis PCC 7937 |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a © Springer Science+Business Media B.V. 2009 | ||
| 520 | |a Abstract Cyanobacteria are rich in polysaccharides and also have a multilayered cell wall that interferes with the successful extraction of RNA. The methods available to extract RNA from cyanobacteria usually give low yield and poor quality of RNA having DNA as contaminant that requires additional treatment with DNase before performing any gene expression studies. Here, we present a simple and rapid method for the extraction of RNA from a filamentous cyanobacterium, Anabaena variabilis PCC 7937, that gives high yield and good quality of RNA without DNA contamination as tested by spectrophotometry, agarose gel electrophoresis, and reverse transcriptase polymerase chain reaction analysis. | ||
| 650 | 4 | |a Agarose gel electrophoresis | |
| 650 | 4 | |a Cyanobacteria | |
| 650 | 4 | |a Reverse transcription-PCR | |
| 650 | 4 | |a RNA extraction | |
| 650 | 4 | |a Spectrophotometry | |
| 700 | 1 | |a Sinha, Rajeshwar P. |4 aut | |
| 700 | 1 | |a Daiker, Viktor |4 aut | |
| 700 | 1 | |a Häder, Donat-P. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of applied phycology |d Springer Netherlands, 1989 |g 22(2009), 1 vom: 19. März, Seite 113-116 |h Online-Ressource |w (DE-627)270429980 |w (DE-600)1477703-4 |w (DE-576)121190390 |x 1573-5176 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:2009 |g number:1 |g day:19 |g month:03 |g pages:113-116 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s10811-009-9428-7 |z lizenzpflichtig |3 Volltext |
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| 952 | |d 22 |j 2009 |e 1 |b 19 |c 03 |h 113-116 | ||