Details der Publikation - Evaluation for Helicobacter pylori in Meckel’s Diverticulum by Using Real-Time PCR

Evaluation for Helicobacter pylori in Meckel’s Diverticulum by Using Real-Time PCR

Background Although Helicobacter pylori (H. pylori) has been identified in heterotopic gastric mucosa of Meckel’s diverticulum, controversial results are reported in the pertinent literature. Aims The aim of this study was to evaluate for the presence of H. pylori histologically using hematoxylin–eosin and Toluidine Blue in Meckel’s diverticulum and by real-time TaqMan polymerase chain reaction (PCR) in those with heterotopic gastric mucosa. Methods The study included 21 consecutive patients who had undergone resection of Meckel’s diverticulum at our hospital between 1995 and 2007. The paraffin-embedded tissues were retrieved and reviewed for the presence of histological abnormalities and H. pylori-like organisms and for the presence or absence of heterotopic mucosa. H. pylori was sought in those cases that contained heterotopic gastric mucosa using real-time TaqMan PCR to amplify a fragment of the 23S ribosomal RNA (rRNA) gene of H. pylori. Results Upon histological examination, heterotopic gastric mucosa was found to be present in 12 cases. H. pylori was not identified in any of the sections examined. A genomic PCR product was also not obtained in real-time PCR study. Conclusions We have confirmed that colonization of H. pylori, if it occurs at all, is exceedingly rare in heterotopic gastric mucosa of Meckel’s diverticulum..

Medienart:	E-Artikel

Erscheinungsjahr:	2009
Erschienen:	2009

Enthalten in:	Zur Gesamtaufnahme - volume:55
Enthalten in:	Digestive diseases and sciences - 55(2009), 7 vom: 29. Aug., Seite 1969-1974

Sprache:	Englisch

Beteiligte Personen:	Tuzun, Ahmet [VerfasserIn] Polat, Zulfikar [VerfasserIn] Kilciler, Guldem [VerfasserIn] Turan, Ilker [VerfasserIn] Kilic, Abdullah [VerfasserIn] Ozcan, Ayhan [VerfasserIn] Uygun, Ahmet [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	44.87$jGastroenterologie
Themen:	Meckel’s diverticulum Real-time polymerase chain reaction

Anmerkungen:	© Springer Science+Business Media, LLC 2009

doi:	10.1007/s10620-009-0958-2

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2112754114

Internformat


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520			\|a Background Although Helicobacter pylori (H. pylori) has been identified in heterotopic gastric mucosa of Meckel’s diverticulum, controversial results are reported in the pertinent literature. Aims The aim of this study was to evaluate for the presence of H. pylori histologically using hematoxylin–eosin and Toluidine Blue in Meckel’s diverticulum and by real-time TaqMan polymerase chain reaction (PCR) in those with heterotopic gastric mucosa. Methods The study included 21 consecutive patients who had undergone resection of Meckel’s diverticulum at our hospital between 1995 and 2007. The paraffin-embedded tissues were retrieved and reviewed for the presence of histological abnormalities and H. pylori-like organisms and for the presence or absence of heterotopic mucosa. H. pylori was sought in those cases that contained heterotopic gastric mucosa using real-time TaqMan PCR to amplify a fragment of the 23S ribosomal RNA (rRNA) gene of H. pylori. Results Upon histological examination, heterotopic gastric mucosa was found to be present in 12 cases. H. pylori was not identified in any of the sections examined. A genomic PCR product was also not obtained in real-time PCR study. Conclusions We have confirmed that colonization of H. pylori, if it occurs at all, is exceedingly rare in heterotopic gastric mucosa of Meckel’s diverticulum.
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912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_230
912			\|a GBV_ILN_267
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_711
912			\|a GBV_ILN_2001
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912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2136
912			\|a GBV_ILN_2143
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912			\|a GBV_ILN_4336
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Evaluation for Helicobacter pylori in Meckel’s Diverticulum by Using Real-Time PCR

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