Details der Publikation - Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death

Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death

Abstract Taxodione, a diterpenoid from the roots of Salvia chorassanica Bunge, possesses cytotoxic, apoptotic, and antimicrobial activity. This study was designed to investigate the protective effects of taxodione on serum/glucose deprivation-induced ischemic injury in PC12 cells and related mechanisms. In an in vitro model of ischemia, PC12 cells were exposed to serum and glucose deprivation for 6 and 18 h. The protective effects of the methanol extract of S. chorassanica and taxodione were assessed using $ alamarBlue^{®} $ assay. Intracellular ROS production was measured by fluorimetry using 2′,7′-dichlorofluorescin diacetate (DCFH-DA). The levels of PARP, Bcl-2, and Bax proteins were detected after western blot analysis. It was shown that taxodione (0.2–1.5 μM) significantly increased cell viability in a dose-dependent manner after ischemic insult. Taxodione has antioxidant activity and protects PC12 cells against oxidative stress-induced apoptotic cell death. Meanwhile, pretreatment with taxodione significantly induced an increase in Bcl-2 and a decrease in Bax protein level. The results of this study confirmed the protective effect of taxodione in serum/glucose deprivation-induced ischemic injury and the putative role of apoptosis as a underling mechanisms. Thus, it would be fair to consider taxodione as a promising ingredient of S. chorassanica for the expansion on novel class of anti-ischemic agents..

Medienart:	E-Artikel

Erscheinungsjahr:	2014
Erschienen:	2014

Enthalten in:	Zur Gesamtaufnahme - volume:34
Enthalten in:	Cellular and molecular neurobiology - 34(2014), 8 vom: 04. Sept., Seite 1103-1109

Sprache:	Englisch

Beteiligte Personen:	Shafaei-Bajestani, Negar [VerfasserIn] Emami, Seyed Ahmad [VerfasserIn] Asili, Javad [VerfasserIn] Tayarani-Najaran, Zahra [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	44.90$jNeurologie
Themen:	Bunge DCFH-DA Lamiaceae PC12 Taxodione

Anmerkungen:	© Springer Science+Business Media New York 2014

doi:	10.1007/s10571-014-0085-2

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2112259660

Internformat


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520			\|a Abstract Taxodione, a diterpenoid from the roots of Salvia chorassanica Bunge, possesses cytotoxic, apoptotic, and antimicrobial activity. This study was designed to investigate the protective effects of taxodione on serum/glucose deprivation-induced ischemic injury in PC12 cells and related mechanisms. In an in vitro model of ischemia, PC12 cells were exposed to serum and glucose deprivation for 6 and 18 h. The protective effects of the methanol extract of S. chorassanica and taxodione were assessed using $ alamarBlue^{®} $ assay. Intracellular ROS production was measured by fluorimetry using 2′,7′-dichlorofluorescin diacetate (DCFH-DA). The levels of PARP, Bcl-2, and Bax proteins were detected after western blot analysis. It was shown that taxodione (0.2–1.5 μM) significantly increased cell viability in a dose-dependent manner after ischemic insult. Taxodione has antioxidant activity and protects PC12 cells against oxidative stress-induced apoptotic cell death. Meanwhile, pretreatment with taxodione significantly induced an increase in Bcl-2 and a decrease in Bax protein level. The results of this study confirmed the protective effect of taxodione in serum/glucose deprivation-induced ischemic injury and the putative role of apoptosis as a underling mechanisms. Thus, it would be fair to consider taxodione as a promising ingredient of S. chorassanica for the expansion on novel class of anti-ischemic agents.
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Anti-apoptotic Effect of Taxodione on Serum/Glucose Deprivation-Induced PC12 Cells Death

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