Details der Publikation - Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate

Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate

Objectives To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate. Results Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1–4. Antioxidative peptides were separated by sequential acid precipitation, cation exchange, and reversed-phase fast performance liquid chromatography. Finally, a peptide with antioxidative activity was identified as Ser-Asn-Leu-Cys-Arg-Pro-Cys-Gly by MALDI time-of-flight (TOF)/TOF analysis, and determined to represent a portion of feather keratin near its N-terminal. A synthesized peptide with the same sequence was used to characterize its antioxidative properties, including scavenging free radicals, reducing power, and $ Fe^{2+} $ chelation. In terms of the peptide’s amino acid composition, the antioxidative activity might be mainly attributed to Cys and other amino acid residues. Conclusion Feather keratin is a good source for the quantitative preparation of antioxidative peptides..

Medienart:	E-Artikel

Erscheinungsjahr:	2015
Erschienen:	2015

Enthalten in:	Zur Gesamtaufnahme - volume:38
Enthalten in:	Biotechnology letters - 38(2015), 4 vom: 19. Dez., Seite 643-649

Sprache:	Englisch

Beteiligte Personen:	Wan, Min-Yuan [VerfasserIn] Dong, Ge [VerfasserIn] Yang, Bin-Qing [VerfasserIn] Feng, Hong [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	42.30$jMikrobiologie 58.30$jBiotechnologie
Themen:	Antioxidative peptide Degradation Feather Hydrolysate Keratin

Anmerkungen:	© Springer Science+Business Media Dordrecht 2015

doi:	10.1007/s10529-015-2016-9

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2111680710

Internformat


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245	1	0	\|a Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate
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520			\|a Objectives To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate. Results Feather hydrolysate was prepared by fermentation with Bacillus subtilis S1–4. Antioxidative peptides were separated by sequential acid precipitation, cation exchange, and reversed-phase fast performance liquid chromatography. Finally, a peptide with antioxidative activity was identified as Ser-Asn-Leu-Cys-Arg-Pro-Cys-Gly by MALDI time-of-flight (TOF)/TOF analysis, and determined to represent a portion of feather keratin near its N-terminal. A synthesized peptide with the same sequence was used to characterize its antioxidative properties, including scavenging free radicals, reducing power, and $ Fe^{2+} $ chelation. In terms of the peptide’s amino acid composition, the antioxidative activity might be mainly attributed to Cys and other amino acid residues. Conclusion Feather keratin is a good source for the quantitative preparation of antioxidative peptides.
650		4	\|a Antioxidative peptide
650		4	\|a Degradation
650		4	\|a Feather
650		4	\|a Hydrolysate
650		4	\|a Keratin
700	1		\|a Dong, Ge \|4 aut
700	1		\|a Yang, Bin-Qing \|4 aut
700	1		\|a Feng, Hong \|4 aut
773	0	8	\|i Enthalten in \|t Biotechnology letters \|d Springer Netherlands, 1979 \|g 38(2015), 4 vom: 19. Dez., Seite 643-649 \|h Online-Ressource \|w (DE-627)312808356 \|w (DE-600)2012203-2 \|w (DE-576)107586002 \|x 1573-6776 \|7 nnns
773	1	8	\|g volume:38 \|g year:2015 \|g number:4 \|g day:19 \|g month:12 \|g pages:643-649
856	4	0	\|u https://dx.doi.org/10.1007/s10529-015-2016-9 \|z lizenzpflichtig \|3 Volltext
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Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate

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