Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions
Abstract Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:83 |
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| Enthalten in: |
Journal of general plant pathology - 83(2016), 1 vom: 01. Dez., Seite 1-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Miyake, Noriyuki [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
Bait trap |
| Anmerkungen: |
© The Phytopathological Society of Japan and Springer Japan 2016 |
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| doi: |
10.1007/s10327-016-0691-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2110192046 |
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| 100 | 1 | |a Miyake, Noriyuki |e verfasserin |0 (orcid)0000-0003-2685-1121 |4 aut | |
| 245 | 1 | 0 | |a Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 520 | |a Abstract Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium-selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P. helicoides in hydroponic culture systems. | ||
| 650 | 4 | |a Bait trap | |
| 650 | 4 | |a Loop-mediated isothermal amplification | |
| 650 | 4 | |a Hydroponic culture system | |
| 650 | 4 | |a Monitoring | |
| 650 | 4 | |a Practical detection method | |
| 700 | 1 | |a Nagai, Hirofumi |4 aut | |
| 700 | 1 | |a Kato, Shinro |4 aut | |
| 700 | 1 | |a Matsusaki, Masashi |4 aut | |
| 700 | 1 | |a Fukuta, Shiro |4 aut | |
| 700 | 1 | |a Takahashi, Reiko |4 aut | |
| 700 | 1 | |a Suzuki, Ryoji |4 aut | |
| 700 | 1 | |a Ishiguro, Yasushi |4 aut | |
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| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s10327-016-0691-6 |z lizenzpflichtig |3 Volltext |
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