Details der Publikation - Genome shuffling of Lactobacillus brevis for enhanced production of thymidine phosphorylase

Genome shuffling of Lactobacillus brevis for enhanced production of thymidine phosphorylase

Abstract Thymidine phosphorylase (TPase) plays a vital role in the biosynthesis of nucleosides and their analogs which have tremendous potential in antiviral and anticancer therapies. In this study, genome shuffling was applied to develop new strains of Lactobacillus brevis with an enhanced production of TPase. The parent organisms were mutated using ultraviolet (UV) irradiation and were shuffled by recursive pool-wise protoplast fusion. The parent protoplasts of each cycle were inactivated by UV irritation for 50 min or by heating at 60°C for 60 min. A rapid and efficient pre-screening method for determining L. brevis fusants with increased TPase production was established by adding appropriate concentrations of substrate thymidine and potassium phosphate to the culture broth based on significant differences in the absorption spectra of substrate thymidine and its product, thymine, in alkaline solution at 290 nm. Strains F3-19 and F3-36 showed high TPase activity and favorable hereditary stability and were screened out through three rounds of recursive protoplast fusion. The increase in the TPase activity of F3-19 and F3-36 was 252.6 and 260.5%, respectively, in comparison with the wild type..

Medienart:	E-Artikel

Erscheinungsjahr:	2015
Erschienen:	2015

Enthalten in:	Zur Gesamtaufnahme - volume:20
Enthalten in:	Biotechnology and bioprocess engineering - 20(2015), 2 vom: Apr., Seite 333-340

Sprache:	Englisch

Beteiligte Personen:	Li, Hongmei [VerfasserIn] Xue, Fang [VerfasserIn] Wang, Weijie [VerfasserIn] Chen, Baozhen [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Genome shuffling Strain screening method Thymidine phosphorylase

Anmerkungen:	© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2015

doi:	10.1007/s12257-014-0617-0

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2102028984

Internformat


LEADER	01000naa a22002652 4500
001	OLC2102028984
003	DE-627
005	20230402024927.0
007	cr uuu---uuuuu
008	230402s2015 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1007/s12257-014-0617-0 \|2 doi
035			\|a (DE-627)OLC2102028984
035			\|a (DE-He213)s12257-014-0617-0-e
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
082	0	4	\|a 570 \|a 690 \|q VZ
100	1		\|a Li, Hongmei \|e verfasserin \|4 aut
245	1	0	\|a Genome shuffling of Lactobacillus brevis for enhanced production of thymidine phosphorylase
264		1	\|c 2015
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
500			\|a © The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2015
520			\|a Abstract Thymidine phosphorylase (TPase) plays a vital role in the biosynthesis of nucleosides and their analogs which have tremendous potential in antiviral and anticancer therapies. In this study, genome shuffling was applied to develop new strains of Lactobacillus brevis with an enhanced production of TPase. The parent organisms were mutated using ultraviolet (UV) irradiation and were shuffled by recursive pool-wise protoplast fusion. The parent protoplasts of each cycle were inactivated by UV irritation for 50 min or by heating at 60°C for 60 min. A rapid and efficient pre-screening method for determining L. brevis fusants with increased TPase production was established by adding appropriate concentrations of substrate thymidine and potassium phosphate to the culture broth based on significant differences in the absorption spectra of substrate thymidine and its product, thymine, in alkaline solution at 290 nm. Strains F3-19 and F3-36 showed high TPase activity and favorable hereditary stability and were screened out through three rounds of recursive protoplast fusion. The increase in the TPase activity of F3-19 and F3-36 was 252.6 and 260.5%, respectively, in comparison with the wild type.
650		4	\|a genome shuffling
650		4	\|a strain screening method
650		4	\|a thymidine phosphorylase
700	1		\|a Xue, Fang \|4 aut
700	1		\|a Wang, Weijie \|4 aut
700	1		\|a Chen, Baozhen \|4 aut
773	0	8	\|i Enthalten in \|t Biotechnology and bioprocess engineering \|d The Korean Society for Biotechnology and Bioengineering, 1996 \|g 20(2015), 2 vom: Apr., Seite 333-340 \|h Online-Ressource \|w (DE-627)373321821 \|w (DE-600)2125481-3 \|w (DE-576)285089153 \|x 1976-3816 \|7 nnns
773	1	8	\|g volume:20 \|g year:2015 \|g number:2 \|g month:04 \|g pages:333-340
856	4	0	\|u https://dx.doi.org/10.1007/s12257-014-0617-0 \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_OLC
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2134
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2474
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 20 \|j 2015 \|e 2 \|c 04 \|h 333-340

Genome shuffling of Lactobacillus brevis for enhanced production of thymidine phosphorylase

Zugang & Verfügbarkeit

Zugehörige Publikationen/Bände