Chimeric epitope vaccine against Leptospira interrogans infection and induced specific immunity in guinea pigs
Background Leptospirosis is an important reemerging zoonosis, with more than half a million cases reported annually, and is caused by pathogenic Leptospira species. Development of a universal vaccine is one of the major strategic goals to overcome the disease burden of leptospirosis. In this study, a chimeric multi-epitope protein-based vaccine was designed and tested for its potency to induce a specific immune response and provide protection against L. interrogans infection. Results The protein, containing four repeats of six T- and B-cell combined epitopes from the leptospiral outer membrane proteins, OmpL1, LipL32 and LipL21, was expressed and purified. Western blot analysis showed that the recombinant protein (named r4R) mainly expressed in a soluble pattern, and reacted with antibodies raised in rabbit against heat-killed Leptospira and in guinea pigs against the r4R vaccine. Microscopic agglutination tests showed that r4R antisera was immunological cross-reactive with a range of Chinese standard reference strains of Leptospira belonging to different serogroups. In guinea pigs, the r4R vaccine induced a Th1-biased immune response, as reflected by the IgG2a/IgG1 ratio and cytokine production of stimulated splenocytes derived from immunized animals. Finally, r4R-immunized guinea pigs showed increased survival of lethal Leptospira challenges compared with PBS-immunized animals and tissue damage and leptospiral colonization of the kidney were reduced. Conclusions The multi-epitope chimeric r4R protein is a promising antigen for the development of a universal cross-reactive vaccine against leptospirosis..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
BMC microbiology - 16(2016), 1 vom: 14. Okt. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lin, Xu’ai [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
Cross protection |
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| Anmerkungen: |
© The Author(s). 2016 |
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| doi: |
10.1186/s12866-016-0852-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2101182149 |
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| 245 | 1 | 0 | |a Chimeric epitope vaccine against Leptospira interrogans infection and induced specific immunity in guinea pigs |
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| 500 | |a © The Author(s). 2016 | ||
| 520 | |a Background Leptospirosis is an important reemerging zoonosis, with more than half a million cases reported annually, and is caused by pathogenic Leptospira species. Development of a universal vaccine is one of the major strategic goals to overcome the disease burden of leptospirosis. In this study, a chimeric multi-epitope protein-based vaccine was designed and tested for its potency to induce a specific immune response and provide protection against L. interrogans infection. Results The protein, containing four repeats of six T- and B-cell combined epitopes from the leptospiral outer membrane proteins, OmpL1, LipL32 and LipL21, was expressed and purified. Western blot analysis showed that the recombinant protein (named r4R) mainly expressed in a soluble pattern, and reacted with antibodies raised in rabbit against heat-killed Leptospira and in guinea pigs against the r4R vaccine. Microscopic agglutination tests showed that r4R antisera was immunological cross-reactive with a range of Chinese standard reference strains of Leptospira belonging to different serogroups. In guinea pigs, the r4R vaccine induced a Th1-biased immune response, as reflected by the IgG2a/IgG1 ratio and cytokine production of stimulated splenocytes derived from immunized animals. Finally, r4R-immunized guinea pigs showed increased survival of lethal Leptospira challenges compared with PBS-immunized animals and tissue damage and leptospiral colonization of the kidney were reduced. Conclusions The multi-epitope chimeric r4R protein is a promising antigen for the development of a universal cross-reactive vaccine against leptospirosis. | ||
| 650 | 4 | |a Leptospira | |
| 650 | 4 | |a Outer membrane protein | |
| 650 | 4 | |a Multi-epitope | |
| 650 | 4 | |a Vaccine | |
| 650 | 4 | |a Cross protection | |
| 700 | 1 | |a Xiao, Guohui |4 aut | |
| 700 | 1 | |a Luo, Dongjiao |4 aut | |
| 700 | 1 | |a Kong, Liangliang |4 aut | |
| 700 | 1 | |a Chen, Xu |4 aut | |
| 700 | 1 | |a Sun, Dexter |4 aut | |
| 700 | 1 | |a Yan, Jie |4 aut | |
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