A sensitive haemadsorption technique based RT-PCR for concentration and detection of Newcastle disease virus from clinical samples and allantoic fluid

Abstract The present study describes the exploitation of haemadsorption (HAd) property of the Newcastle disease virus (NDV) for the development of a novel sensitive HAd technique based RT-PCR for detection of NDV from clinical samples of virus infected experimental birds. The NDV propagated allantoic fluid from the infected embryonated chicken eggs or supernatant of the processed clinical samples (tissue triturate, cloaca and tracheal swabs) from the experimentally infected birds were added with chicken red blood cells (RBC) to adsorb the virus on RBC’s surface. The virus adsorbed RBCs were subjected to trizol method of RNA extraction and reverse transcription-polymerase chain reaction (RT-PCR) for detection of NDV. The HAd based RNA extraction showed better yield of 700–900 ng RNA and when subjected to RT-PCR detection revealed a 100 times higher sensitivity than the conventional RNA extraction and RT-PCR detection system. This could be an alternate technique which can be exploited in low NDV load situations in clinical samples..

Medienart:

E-Artikel

Erscheinungsjahr:

2016

Erschienen:

2016

Enthalten in:

Zur Gesamtaufnahme - volume:27

Enthalten in:

VirusDisease - 27(2016), 3 vom: 11. Juni, Seite 319-323

Sprache:

Englisch

Beteiligte Personen:

Desingu, Perumal Arumugam [VerfasserIn]
Singh, Shambhu Dayal [VerfasserIn]
Dhama, Kuldeep [VerfasserIn]
Vinodhkumar, Obli Rajendran [VerfasserIn]
Malik, Yashpal Singh [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

Themen:

Haemadsorption
Newcastle disease
RNA extraction
RT-PCR

Anmerkungen:

© Indian Virological Society 2016

doi:

10.1007/s13337-016-0325-9

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC209761583X

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