Mineralization of 4-fluorocinnamic acid by a Rhodococcus strain

Abstract A bacterial strain capable of aerobic degradation of 4-fluorocinnamic acid (4-FCA) as the sole source of carbon and energy was isolated from a biofilm reactor operating for the treatment of 2-fluorophenol. The organism, designated as strain S2, was identified by 16S rRNA gene analysis as a member of the genus Rhodococcus. Strain S2 was able to mineralize 4-FCA as sole carbon and energy source. In the presence of a conventional carbon source (sodium acetate [SA]), growth rate of strain S2 was enhanced from 0.04 to 0.14 $ h^{−1} $ when the culture medium was fed with 0.5 mM of 4-FCA, and the time for complete removal of 4-FCA decreased from 216 to 50 h. When grown in SA-supplemented medium, 4-FCA concentrations up to 1 mM did not affect the length of the lag phase, and for 4-FCA concentrations up to 3 mM, strain S2 was able to completely remove the target fluorinated compound. 4-Fluorobenzoate (4-FBA) was transiently formed in the culture medium, reaching concentrations up to 1.7 mM when the cultures were supplemented with 3.5 mM of 4-FCA. Trans,trans-muconate was also transiently formed as a metabolic intermediate. Compounds with molecular mass compatible with 3-carboxymuconate and 3-oxoadipate were also detected in the culture medium. Strain S2 was able to mineralize a range of other haloorganic compounds, including 2-fluorophenol, to which the biofilm reactor had been exposed. To our knowledge, this is the first time that mineralization of 4-FCA as the sole carbon source by a single bacterial culture is reported..

Medienart:

E-Artikel

Erscheinungsjahr:

2013

Erschienen:

2013

Enthalten in:

Zur Gesamtaufnahme - volume:98

Enthalten in:

Applied microbiology and biotechnology - 98(2013), 4 vom: 15. Aug., Seite 1893-1905

Sprache:

Englisch

Beteiligte Personen:

Amorim, Catarina L. [VerfasserIn]
Ferreira, António C. S. [VerfasserIn]
Carvalho, Maria F. [VerfasserIn]
Afonso, Carlos M. M. [VerfasserIn]
Castro, Paula M. L. [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

BKL:

58.30$jBiotechnologie

42.30$jMikrobiologie

Themen:

4-Fluorocinnamic acid
Biodegradation
Metabolism
Sp

RVK:

RVK Klassifikation

Anmerkungen:

© Springer-Verlag Berlin Heidelberg 2013

doi:

10.1007/s00253-013-5149-6

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2091103934

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