Details der Publikation - An ELISA based on the repeated foot-and-mouth disease virus 3B epitope peptide can distinguish infected and vaccinated cattle

An ELISA based on the repeated foot-and-mouth disease virus 3B epitope peptide can distinguish infected and vaccinated cattle

Abstract To develop a strategy of differentiating infected from vaccinated animals (DIVA) with foot-and-mouth disease virus (FMDV), a short (27aa) peptide containing three conserved linear B cell epitopes of the FMDV 3B nonstructural protein was designed. This novel BF peptide was synthesized using a gene splicing by overlap extension protocol with preferred codons for Escherichia coli. The resultant eight tandem repeat multimer (1, 2, 4, 6, 8, 16, 24, and 32BF) were expressed as soluble fusion proteins in E. coli. An indirect ELISA was developed based on the recombinant 8BF protein with the aim of specifically distinguishing antibodies induced by FMDV infection but not those induced by vaccination. Using the cut-off value of 0.3, the sensitivity of the assay was 96.8% and the specificities for naive and vaccinated cattle were 99.8 and 99.0%, respectively. The performance of the newly developed epitope-based ELISA was compared with three commercial NSP ELISA kits. The 8BF-ELISA appears to be a promising DIVA test for FMD control and eradication..

Medienart:	E-Artikel

Erscheinungsjahr:	2011
Erschienen:	2011

Enthalten in:	Zur Gesamtaufnahme - volume:93
Enthalten in:	Applied microbiology and biotechnology - 93(2011), 3 vom: 30. Dez., Seite 1271-1279

Sprache:	Englisch

Beteiligte Personen:	Gao, Mingchun [VerfasserIn] Zhang, Runxiang [VerfasserIn] Li, Meng [VerfasserIn] Li, Shuang [VerfasserIn] Cao, Yongsheng [VerfasserIn] Ma, Bo [VerfasserIn] Wang, Junwei [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	58.30$jBiotechnologie 42.30$jMikrobiologie
Themen:	DIVA ELISA Epitope Foot-and-mouth disease virus Tandem repeat

RVK:	RVK Klassifikation ELIB09 ELIB10 ELIB24 ELIB41 ELIB22

Anmerkungen:	© Springer-Verlag 2011

doi:	10.1007/s00253-011-3815-0

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC209108204X

Internformat


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245	1	0	\|a An ELISA based on the repeated foot-and-mouth disease virus 3B epitope peptide can distinguish infected and vaccinated cattle
264		1	\|c 2011
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520			\|a Abstract To develop a strategy of differentiating infected from vaccinated animals (DIVA) with foot-and-mouth disease virus (FMDV), a short (27aa) peptide containing three conserved linear B cell epitopes of the FMDV 3B nonstructural protein was designed. This novel BF peptide was synthesized using a gene splicing by overlap extension protocol with preferred codons for Escherichia coli. The resultant eight tandem repeat multimer (1, 2, 4, 6, 8, 16, 24, and 32BF) were expressed as soluble fusion proteins in E. coli. An indirect ELISA was developed based on the recombinant 8BF protein with the aim of specifically distinguishing antibodies induced by FMDV infection but not those induced by vaccination. Using the cut-off value of 0.3, the sensitivity of the assay was 96.8% and the specificities for naive and vaccinated cattle were 99.8 and 99.0%, respectively. The performance of the newly developed epitope-based ELISA was compared with three commercial NSP ELISA kits. The 8BF-ELISA appears to be a promising DIVA test for FMD control and eradication.
650		4	\|a Foot-and-mouth disease virus
650		4	\|a Tandem repeat
650		4	\|a Epitope
650		4	\|a ELISA
650		4	\|a DIVA
700	1		\|a Zhang, Runxiang \|4 aut
700	1		\|a Li, Meng \|4 aut
700	1		\|a Li, Shuang \|4 aut
700	1		\|a Cao, Yongsheng \|4 aut
700	1		\|a Ma, Bo \|4 aut
700	1		\|a Wang, Junwei \|4 aut
773	0	8	\|i Enthalten in \|t Applied microbiology and biotechnology \|d Springer-Verlag, 1975 \|g 93(2011), 3 vom: 30. Dez., Seite 1271-1279 \|h Online-Ressource \|w (DE-627)265509564 \|w (DE-600)1464336-4 \|w (DE-576)074889737 \|x 1432-0614 \|7 nnns
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An ELISA based on the repeated foot-and-mouth disease virus 3B epitope peptide can distinguish infected and vaccinated cattle

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