Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response
Abstract Lactococcus lactis is a potential host for production of recombinant proteins, especially of therapeutic importance. However, in glucose-grown cultures, lowering of pH due to accumulation of lactic acid and the concomitant induction of acid tolerance response (ATR) may affect the recombinant protein produced. In this work, we have analyzed the effect of culture pH and the associated ATR on production of recombinant streptokinase. Streptokinase gene was cloned and expressed as a secretory protein in L. lactis under the control of P170 promoter. It was found to undergo degradation to form inactive products leading to low productivity. The extent of degradation and productivity of streptokinase was greatly influenced by the development of ATR, which was dependent on the pH of the culture and initial phosphate concentration of the medium. It was found that high pH and high initial phosphate concentration leads to suppression of ATR and this results in at least 2.5-fold increase in streptokinase productivity and significant decrease in degradation of streptokinase..
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2006 |
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| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
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| Enthalten in: |
Applied microbiology and biotechnology - 72(2006), 6 vom: 01. Apr., Seite 1202-1209 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sriraman, Kalpana [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Early Stationary Phase |
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| Anmerkungen: |
© Springer-Verlag 2006 |
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| doi: |
10.1007/s00253-006-0410-x |
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| 245 | 1 | 0 | |a Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response |
| 264 | 1 | |c 2006 | |
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| 520 | |a Abstract Lactococcus lactis is a potential host for production of recombinant proteins, especially of therapeutic importance. However, in glucose-grown cultures, lowering of pH due to accumulation of lactic acid and the concomitant induction of acid tolerance response (ATR) may affect the recombinant protein produced. In this work, we have analyzed the effect of culture pH and the associated ATR on production of recombinant streptokinase. Streptokinase gene was cloned and expressed as a secretory protein in L. lactis under the control of P170 promoter. It was found to undergo degradation to form inactive products leading to low productivity. The extent of degradation and productivity of streptokinase was greatly influenced by the development of ATR, which was dependent on the pH of the culture and initial phosphate concentration of the medium. It was found that high pH and high initial phosphate concentration leads to suppression of ATR and this results in at least 2.5-fold increase in streptokinase productivity and significant decrease in degradation of streptokinase. | ||
| 650 | 4 | |a Streptokinase | |
| 650 | 4 | |a Phosphate Concentration | |
| 650 | 4 | |a P170 Promoter | |
| 650 | 4 | |a Recombinant Protein Production | |
| 650 | 4 | |a Early Stationary Phase | |
| 700 | 1 | |a Jayaraman, Guhan |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Springer Berlin Heidelberg, 1975 |g 72(2006), 6 vom: 01. Apr., Seite 1202-1209 |h Online-Ressource |w (DE-627)265509564 |w (DE-600)1464336-4 |w (DE-576)074889737 |x 1432-0614 |7 nnns |
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