Affinity capillary electrophoresis for identification of active drug candidates in myotonic dystrophy type 1
Abstract Myotonic dystrophy type 1 (DM1) is an autosomal dominantly inherited degenerative disease with a slow progression. At the present, there is no commercially available treatment, but sustained effort is currently undertaken for the development of a promising lead compound. In the present paper we report the development of a fast, versatile, and cost-effective affinity capillary electrophoresis (ACE) method for the screening and identification of potential drug candidates targeting pathological ARN probes relevant for DM1. The affinity studies were conducted in physiologically relevant conditions using 50 mM HEPES buffer (pH 7.4) in a fused silica capillary dynamically coated with poly(ethylene oxide), by testing a library of potential ligands against (CUG)50 RNA as target probe with a total run time of 4–5 h/ligand. For the most promising ligands, their affinity parameters were assessed and some results formerly reported on the affinity of pentamidine (PTMD) and neomycin against CUG repeats were confirmed. To the best of the authors’ knowledge, the estimated binding stoichiometry for some of the tested compounds (i.e., ~ 121:1 for PTMD against the tested RNA probe) is reported for the first time. Additionally, the potential of a novel pentamidine like compound, namely 1,2-ethane bis-1-amino-4-benzamidine (EBAB) with much lower in vivo toxicity than its parent compound has also been confirmed studying its effect on a live cell model by fluorescence microscopy. Further tests, such as the evaluation of the rescue in the mis-splicing of the involved genes, can be performed to corroborate the potential therapeutic value of EBAB in DM1 treatment. Graphical abstractᅟ.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:410 |
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| Enthalten in: |
Analytical & bioanalytical chemistry - 410(2018), 18 vom: 08. Mai, Seite 4495-4507 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Neaga, Ioan O. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Affinity capillary electrophoresis |
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| Anmerkungen: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2018 |
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| doi: |
10.1007/s00216-018-1107-6 |
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| 520 | |a Abstract Myotonic dystrophy type 1 (DM1) is an autosomal dominantly inherited degenerative disease with a slow progression. At the present, there is no commercially available treatment, but sustained effort is currently undertaken for the development of a promising lead compound. In the present paper we report the development of a fast, versatile, and cost-effective affinity capillary electrophoresis (ACE) method for the screening and identification of potential drug candidates targeting pathological ARN probes relevant for DM1. The affinity studies were conducted in physiologically relevant conditions using 50 mM HEPES buffer (pH 7.4) in a fused silica capillary dynamically coated with poly(ethylene oxide), by testing a library of potential ligands against (CUG)50 RNA as target probe with a total run time of 4–5 h/ligand. For the most promising ligands, their affinity parameters were assessed and some results formerly reported on the affinity of pentamidine (PTMD) and neomycin against CUG repeats were confirmed. To the best of the authors’ knowledge, the estimated binding stoichiometry for some of the tested compounds (i.e., ~ 121:1 for PTMD against the tested RNA probe) is reported for the first time. Additionally, the potential of a novel pentamidine like compound, namely 1,2-ethane bis-1-amino-4-benzamidine (EBAB) with much lower in vivo toxicity than its parent compound has also been confirmed studying its effect on a live cell model by fluorescence microscopy. Further tests, such as the evaluation of the rescue in the mis-splicing of the involved genes, can be performed to corroborate the potential therapeutic value of EBAB in DM1 treatment. Graphical abstractᅟ | ||
| 650 | 4 | |a Myotonic dystrophy type 1 | |
| 650 | 4 | |a Ligand screening | |
| 650 | 4 | |a Affinity capillary electrophoresis | |
| 650 | 4 | |a Fluorescence in situ hybridization | |
| 650 | 4 | |a Pentamidine | |
| 700 | 1 | |a Hambye, Stephanie |4 aut | |
| 700 | 1 | |a Bodoki, Ede |4 aut | |
| 700 | 1 | |a Palmieri, Claudio |4 aut | |
| 700 | 1 | |a Ansseau, Eugénie |4 aut | |
| 700 | 1 | |a Belayew, Alexandra |4 aut | |
| 700 | 1 | |a Oprean, Radu |4 aut | |
| 700 | 1 | |a Blankert, Bertrand |4 aut | |
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