Details der Publikation - Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer

Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer

Abstract MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer..

Medienart:	E-Artikel

Erscheinungsjahr:	2017
Erschienen:	2017

Enthalten in:	Zur Gesamtaufnahme - volume:11
Enthalten in:	Frontiers of medicine - 11(2017), 2 vom: 04. Mai, Seite 214-222

Sprache:	Englisch

Beteiligte Personen:	Zhou, Bo [VerfasserIn] Xu, Hongbin [VerfasserIn] Xia, Meng [VerfasserIn] Sun, Chaoyang [VerfasserIn] Li, Na [VerfasserIn] Guo, Ensong [VerfasserIn] Guo, Lili [VerfasserIn] Shan, Wanying [VerfasserIn] Lu, Hao [VerfasserIn] Wu, Yifan [VerfasserIn] Li, Yuan [VerfasserIn] Yang, Degui [VerfasserIn] Weng, Danhui [VerfasserIn] Meng, Li [VerfasserIn] Hu, Junbo [VerfasserIn] Ma, Ding [VerfasserIn] Chen, Gang [VerfasserIn] Li, Kezhen [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

BKL:	44.00 / Medizin: Allgemeines / Medizin: Allgemeines
Themen:	E-cadherin Metastasis MiR-9 Ovarian cancer

Anmerkungen:	© Higher Education Press and Springer-Verlag Berlin Heidelberg 2017

doi:	10.1007/s11684-017-0518-7

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2088591176

Internformat


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520			\|a Abstract MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer.
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700	1		\|a Xu, Hongbin \|4 aut
700	1		\|a Xia, Meng \|4 aut
700	1		\|a Sun, Chaoyang \|4 aut
700	1		\|a Li, Na \|4 aut
700	1		\|a Guo, Ensong \|4 aut
700	1		\|a Guo, Lili \|4 aut
700	1		\|a Shan, Wanying \|4 aut
700	1		\|a Lu, Hao \|4 aut
700	1		\|a Wu, Yifan \|4 aut
700	1		\|a Li, Yuan \|4 aut
700	1		\|a Yang, Degui \|4 aut
700	1		\|a Weng, Danhui \|4 aut
700	1		\|a Meng, Li \|4 aut
700	1		\|a Hu, Junbo \|4 aut
700	1		\|a Ma, Ding \|4 aut
700	1		\|a Chen, Gang \|4 aut
700	1		\|a Li, Kezhen \|4 aut
773	0	8	\|i Enthalten in \|t Frontiers of medicine \|d Higher Education Press, 2011 \|g 11(2017), 2 vom: 04. Mai, Seite 214-222 \|h Online-Ressource \|w (DE-627)664261027 \|w (DE-600)2617113-2 \|w (DE-576)370704541 \|x 2095-0225 \|7 nnns
773	1	8	\|g volume:11 \|g year:2017 \|g number:2 \|g day:04 \|g month:05 \|g pages:214-222
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951			\|a AR
952			\|d 11 \|j 2017 \|e 2 \|b 04 \|c 05 \|h 214-222

Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer

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