Details der Publikation - Organelle antioxidants improve microspore embryogenesis in wheat and triticale

Organelle antioxidants improve microspore embryogenesis in wheat and triticale

Abstract Low frequency of green plant production and albinism limits the use of isolated microspore culture (IMC) in cereal breeding programs. The present study was conducted in triticale and bread wheat IMC to increase the production of green plants and minimize albinism. NPB-99 + 10% Ficoll induction medium was supplemented with mitochondrial or plastid antioxidants, in a completely random design, to evaluate their contribution to successful microspore embryogenesis and green plant production. Each group of antioxidants was tested independently: first in triticale and then validated in various spring wheat genotypes. While the response differed by wheat genotype, induction medium supplemented with proline (10 mM) yielded a greater number of embryos/embryo-like structures and green plants in both triticale and wheat. No differences were found with respect to albinism in triticale or wheat except for the cv. Sadash. Among plastid antioxidants tested, glutathione (2 μM) proved to be the best antioxidant to increase embryo and green plant production. Salicylic acid also helped to reduce the number of albino plants in triticale and the wheat genotype SWS366. Overall, induction medium supplemented with proline or glutathione enhanced microspore embryogenesis in both triticale and wheat and increased the number of green plants in the recalcitrant genotypes..

Medienart:	E-Artikel

Erscheinungsjahr:	2013
Erschienen:	2013

Enthalten in:	Zur Gesamtaufnahme - volume:49
Enthalten in:	In vitro cellular & developmental biology / Plant - 49(2013), 5 vom: 10. Mai, Seite 489-497

Sprache:	Englisch

Beteiligte Personen:	Asif, Muhammad [VerfasserIn] Eudes, François [VerfasserIn] Goyal, Aakash [VerfasserIn] Amundsen, Eric [VerfasserIn] Randhawa, Harpinder [VerfasserIn] Spaner, Dean [VerfasserIn]

Links:	Volltext [lizenzpflichtig]

Themen:	Antioxidants Chloroplast Microspore culture Mitochondria Triticale Wheat

Anmerkungen:	© The Society for In Vitro Biology 2013

doi:	10.1007/s11627-013-9514-z

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC2088064001

Internformat


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520			\|a Abstract Low frequency of green plant production and albinism limits the use of isolated microspore culture (IMC) in cereal breeding programs. The present study was conducted in triticale and bread wheat IMC to increase the production of green plants and minimize albinism. NPB-99 + 10% Ficoll induction medium was supplemented with mitochondrial or plastid antioxidants, in a completely random design, to evaluate their contribution to successful microspore embryogenesis and green plant production. Each group of antioxidants was tested independently: first in triticale and then validated in various spring wheat genotypes. While the response differed by wheat genotype, induction medium supplemented with proline (10 mM) yielded a greater number of embryos/embryo-like structures and green plants in both triticale and wheat. No differences were found with respect to albinism in triticale or wheat except for the cv. Sadash. Among plastid antioxidants tested, glutathione (2 μM) proved to be the best antioxidant to increase embryo and green plant production. Salicylic acid also helped to reduce the number of albino plants in triticale and the wheat genotype SWS366. Overall, induction medium supplemented with proline or glutathione enhanced microspore embryogenesis in both triticale and wheat and increased the number of green plants in the recalcitrant genotypes.
650		4	\|a Antioxidants
650		4	\|a Chloroplast
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650		4	\|a Microspore culture
650		4	\|a Triticale
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700	1		\|a Amundsen, Eric \|4 aut
700	1		\|a Randhawa, Harpinder \|4 aut
700	1		\|a Spaner, Dean \|4 aut
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Organelle antioxidants improve microspore embryogenesis in wheat and triticale

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