Comparative study of commercial media to improve GMP manufacturing of recombinant human interferon β-1a by CHO cells in perfusion bioreactor
Abstract Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:74 |
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| Enthalten in: |
Cytotechnology - 74(2022), 6 vom: 12. Okt., Seite 669-680 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sedighikamal, Hossein [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Chinese hamster ovary cells |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| doi: |
10.1007/s10616-022-00554-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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OLC2079958704 |
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| 520 | |a Abstract Chinese hamster ovary cells are the main cellular factories for production of a wide range of recombinant proteins in biopharmaceutical industry. Recombinant human Interferon beta-1a (rh-IFN β-1a), as a cytokine is broadly used to treat multiple sclerosis. In this work, the cell line producing rh-IFN β-1a was studied to improve cell density along with the specific expression. For this reason different cell culture experiments were done using different commercial serum-free media to find the appropriate media providing higher cell density. It was shown DMEMF12, DMEM:ProCHO5, and CHO-S-SFM II led to higher cell density and shorter doubling time. Next, using these media, fed-batch, and perfusion culture with temperature shift were implemented to investigate the best condition for industrial-scale manufacturing of rh-IFN β-1a in terms of higher cell density and product expression yield. The results demonstrated that CHO-S-SFM II media and a thermally biphasic condition provide enhanced expression of rh-IFN β-1a in perfusion bioreactor. | ||
| 650 | 4 | |a Chinese hamster ovary cells | |
| 650 | 4 | |a Specific productivity | |
| 650 | 4 | |a Rh-IFN β-1a | |
| 650 | 4 | |a Fed-batch | |
| 650 | 4 | |a Perfusion | |
| 700 | 1 | |a Karimi Mostofi, Reza |4 aut | |
| 700 | 1 | |a Sattarzadeh, Alireza |4 aut | |
| 700 | 1 | |a Shahbazi, Mansour |4 aut | |
| 700 | 1 | |a Aghazadeh, Hossein |4 aut | |
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