Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome
Abstract Thecaphora frezii is a phytopathogenic fungus belonging to the Ustilaginomycetes class, which causes peanut smut disease. In its biological cycle it has three structures, teliospores (is the resistance structure), basidiospores and hyphae. The mycelium is the structure that penetrates the plant’s gynophore and initiates the infection. For this action, the expression of effectors, pathogenicity and virulence factor proteins is necessary. The aim of this study was to identify potential Thecaphora frezii’s proteins that could exert pathogenicity, virulence and/or effector functions. Based on the transcriptome of two ontogenetic stages (teliospore and hyphae) of Thecaphora frezii, and a series of bioinformatic analyses, 18 potential effectors and 91 factors possibly involved in the level of pathogenicity and virulence were identified. Higher expression levels of candidate effectors were found in the infective stage of the fungus. In the other hand, the major variability observed in pathogenicity and virulence factors expressed between Ustilago maydis and Thecaphora frezii were the number of virulence factors secreted (higher in U. maydis). In the future, when the genome of the fungus will be known, functional studies of these proteins could be carried out to validate their function..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:164 |
|---|---|
| Enthalten in: |
European journal of plant pathology - 164(2022), 3 vom: 17. Aug., Seite 325-335 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Soria, Néstor W. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
|---|
| Anmerkungen: |
© Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
|---|
| doi: |
10.1007/s10658-022-02562-2 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2079818562 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2079818562 | ||
| 003 | DE-627 | ||
| 005 | 20230518155838.0 | ||
| 007 | tu | ||
| 008 | 230131s2022 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10658-022-02562-2 |2 doi | |
| 035 | |a (DE-627)OLC2079818562 | ||
| 035 | |a (DE-He213)s10658-022-02562-2-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 580 |a 630 |a 640 |q VZ |
| 084 | |a 12 |2 ssgn | ||
| 100 | 1 | |a Soria, Néstor W. |e verfasserin |0 (orcid)0000-0002-8232-1822 |4 aut | |
| 245 | 1 | 0 | |a Elucidating potential effectors, pathogenicity and virulence factors expressed by the phytopathogenic fungus Thecaphora frezii through analysis of its transcriptome |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. | ||
| 520 | |a Abstract Thecaphora frezii is a phytopathogenic fungus belonging to the Ustilaginomycetes class, which causes peanut smut disease. In its biological cycle it has three structures, teliospores (is the resistance structure), basidiospores and hyphae. The mycelium is the structure that penetrates the plant’s gynophore and initiates the infection. For this action, the expression of effectors, pathogenicity and virulence factor proteins is necessary. The aim of this study was to identify potential Thecaphora frezii’s proteins that could exert pathogenicity, virulence and/or effector functions. Based on the transcriptome of two ontogenetic stages (teliospore and hyphae) of Thecaphora frezii, and a series of bioinformatic analyses, 18 potential effectors and 91 factors possibly involved in the level of pathogenicity and virulence were identified. Higher expression levels of candidate effectors were found in the infective stage of the fungus. In the other hand, the major variability observed in pathogenicity and virulence factors expressed between Ustilago maydis and Thecaphora frezii were the number of virulence factors secreted (higher in U. maydis). In the future, when the genome of the fungus will be known, functional studies of these proteins could be carried out to validate their function. | ||
| 650 | 4 | |a Effectors | |
| 650 | 4 | |a Pathogenicity | |
| 650 | 4 | |a Virulence | |
| 650 | 4 | |a Peanut | |
| 700 | 1 | |a Badariotti, Esteban H. |4 aut | |
| 700 | 1 | |a Alasino, Valeria R. |0 (orcid)0000-0003-2636-2109 |4 aut | |
| 700 | 1 | |a Figueroa, Ana C. |0 (orcid)0000-0002-9567-4192 |4 aut | |
| 700 | 1 | |a Díaz, María S. |0 (orcid)0000-0003-0239-0295 |4 aut | |
| 700 | 1 | |a Yang, Pablo |0 (orcid)0000-0001-5206-8873 |4 aut | |
| 700 | 1 | |a Beltramo, Dante M. |0 (orcid)0000-0002-8455-9068 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of plant pathology |d Springer Netherlands, 1994 |g 164(2022), 3 vom: 17. Aug., Seite 325-335 |w (DE-627)182314359 |w (DE-600)1196520-4 |w (DE-576)041596773 |x 0929-1873 |7 nnns |
| 773 | 1 | 8 | |g volume:164 |g year:2022 |g number:3 |g day:17 |g month:08 |g pages:325-335 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s10658-022-02562-2 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-FOR | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_252 | ||
| 912 | |a GBV_ILN_2404 | ||
| 951 | |a AR | ||
| 952 | |d 164 |j 2022 |e 3 |b 17 |c 08 |h 325-335 | ||