Gallic Acid and Gallic Acid Nanoparticle Modulate Insulin Secretion Pancreatic β-Islets against Silica Nanoparticle–Induced Oxidative Damage
Abstract Due to the increasing use of silica nanoparticles (SiNPs), their possible toxic effects on human health have undoubtedly been considered. Previous studies proved that SiNPs induced oxidative stress. Reactive oxygen species (ROS) and oxidative stress disrupt cell function and decrease insulin secretion. Therefore, this study intended to assess the effects of SiNPs on oxidative stress and insulin secretion and also the protective effects of gallic acid (GA) and gallic acid nanoparticles (NP-GA) on pancreatic β-islets. In this study, the mice islets were separated and pretreated with various concentrations of GA and NP-GA then treated with a single dose of SiNPs. The cell viability of islets examined by MTT assay and also the levels of ROS, malondialdehyde (MDA), glutathione (GSH); activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and insulin secretion were evaluated. The results of MTT assay showed that SiNPs reduced islet viability in a dose-dependent manner and also insulin secretion, induced the formation of ROS, augmented MDA amounts, and decreased GSH levels, SOD, GPx, and CAT activities. Furthermore, pretreatment of islets with GA and NP-GA significantly returned these alterations at low dose. These findings suggested that SiNPs induced oxidative stress in the pancreatic islets, which could be one of the reasons for the decrease in insulin secretion and inducing diabetes. This study also showed that low doses of GA and NP-GA boosted the antioxidant defense system in the pancreatic β-islets, preventing oxidative stress and, consequently, the progression of diabetes..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:200 |
|---|---|
| Enthalten in: |
Biological trace element research - 200(2022), 12 vom: 12. Jan., Seite 5159-5171 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ahangarpour, Akram [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Diabetes |
|---|
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 |
|---|
| doi: |
10.1007/s12011-022-03111-y |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2079729284 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2079729284 | ||
| 003 | DE-627 | ||
| 005 | 20230513020115.0 | ||
| 007 | tu | ||
| 008 | 221221s2022 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12011-022-03111-y |2 doi | |
| 035 | |a (DE-627)OLC2079729284 | ||
| 035 | |a (DE-He213)s12011-022-03111-y-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 570 |q VZ |
| 084 | |a 12 |2 ssgn | ||
| 084 | |a BIODIV |q DE-30 |2 fid | ||
| 100 | 1 | |a Ahangarpour, Akram |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Gallic Acid and Gallic Acid Nanoparticle Modulate Insulin Secretion Pancreatic β-Islets against Silica Nanoparticle–Induced Oxidative Damage |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 | ||
| 520 | |a Abstract Due to the increasing use of silica nanoparticles (SiNPs), their possible toxic effects on human health have undoubtedly been considered. Previous studies proved that SiNPs induced oxidative stress. Reactive oxygen species (ROS) and oxidative stress disrupt cell function and decrease insulin secretion. Therefore, this study intended to assess the effects of SiNPs on oxidative stress and insulin secretion and also the protective effects of gallic acid (GA) and gallic acid nanoparticles (NP-GA) on pancreatic β-islets. In this study, the mice islets were separated and pretreated with various concentrations of GA and NP-GA then treated with a single dose of SiNPs. The cell viability of islets examined by MTT assay and also the levels of ROS, malondialdehyde (MDA), glutathione (GSH); activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and insulin secretion were evaluated. The results of MTT assay showed that SiNPs reduced islet viability in a dose-dependent manner and also insulin secretion, induced the formation of ROS, augmented MDA amounts, and decreased GSH levels, SOD, GPx, and CAT activities. Furthermore, pretreatment of islets with GA and NP-GA significantly returned these alterations at low dose. These findings suggested that SiNPs induced oxidative stress in the pancreatic islets, which could be one of the reasons for the decrease in insulin secretion and inducing diabetes. This study also showed that low doses of GA and NP-GA boosted the antioxidant defense system in the pancreatic β-islets, preventing oxidative stress and, consequently, the progression of diabetes. | ||
| 650 | 4 | |a Silica nanoparticles | |
| 650 | 4 | |a Gallic acid | |
| 650 | 4 | |a Gallic acid nanoparticles | |
| 650 | 4 | |a Diabetes | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a Islet insulin secretion | |
| 700 | 1 | |a Sharifinasab, Hassan |4 aut | |
| 700 | 1 | |a Kalantari, Heibatullah |4 aut | |
| 700 | 1 | |a Dehghani, Mohammad Amin |4 aut | |
| 700 | 1 | |a Maram, Nader Shakiba |4 aut | |
| 700 | 1 | |a Golfakhrabadi, Fereshteh |0 (orcid)0000-0002-0606-2937 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biological trace element research |d Springer US, 1979 |g 200(2022), 12 vom: 12. Jan., Seite 5159-5171 |w (DE-627)130077275 |w (DE-600)445336-0 |w (DE-576)015614395 |x 0163-4984 |7 nnns |
| 773 | 1 | 8 | |g volume:200 |g year:2022 |g number:12 |g day:12 |g month:01 |g pages:5159-5171 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s12011-022-03111-y |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-CHE | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 951 | |a AR | ||
| 952 | |d 200 |j 2022 |e 12 |b 12 |c 01 |h 5159-5171 | ||