Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere
Abstract A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2 → 6) linked backbone with β(2 → 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × $ 10^{7} $ Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:115 |
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| Enthalten in: |
Antonie van Leeuwenhoek - 115(2022), 9 vom: 15. Juli, Seite 1101-1112 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nasir, Anam [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature Switzerland AG 2022 |
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| doi: |
10.1007/s10482-022-01760-6 |
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| PPN (Katalog-ID): |
OLC2079316117 |
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| 520 | |a Abstract A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2 → 6) linked backbone with β(2 → 1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × $ 10^{7} $ Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications. | ||
| 650 | 4 | |a Exopolysaccharide | |
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| 650 | 4 | |a Fructooligosaccharide | |
| 650 | 4 | |a Levan | |
| 700 | 1 | |a Ahmad, Waqar |4 aut | |
| 700 | 1 | |a Sattar, Fazal |4 aut | |
| 700 | 1 | |a Ashfaq, Iram |4 aut | |
| 700 | 1 | |a Lindemann, Stephen R. |4 aut | |
| 700 | 1 | |a Chen, Ming-Hsu |4 aut | |
| 700 | 1 | |a Van den Ende, Wim |4 aut | |
| 700 | 1 | |a Ӧner, Ebru Toksoy |4 aut | |
| 700 | 1 | |a Kirtel, Onur |4 aut | |
| 700 | 1 | |a Khaliq, Shazia |4 aut | |
| 700 | 1 | |a Ghauri, Muhammad A. |4 aut | |
| 700 | 1 | |a Anwar, Munir A. |0 (orcid)0000-0002-4443-1406 |4 aut | |
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