Recombinant Expression and Antibacterial Properties of BmTXKS2 Venom Peptide in Fusion with GST
Abstract The increase in antibiotic-resistant bacteria cases is an immediate global health problem. Scorpion venom is an excellent candidate for new antimicrobial drug development due to its ability to prevent drug resistance with fewer side effects. BmTXKS2 is a venom peptide identified from a full-length cDNA clone from the venom gland of the Chinese scorpion, Buthus martensii Karsch. It is predicted to have 39 residues with three disulfide bridges and have similarity to hemolymph defensins. In this work, GST-His-BmTXKS2 plasmid was chemically synthesized, inserted into vector pGEX-4T-1, and expressed in Escherichia coli (E. coli). The glutathione-S-transferase (GST) fusion product of BmTXKS2 in a pure form was expressed in a sufficient amount to characterize its antibacterial activity which was assayed through the minimum inhibitory concentration (MIC) method and inhibition zone assay. GST-His-BmTXKS2 exhibited potent inhibitory effects on the four strains (Gram-negative bacteria with a MIC of 12.5–13 μg/mL for E. coli ATCC 25922, 18–19.5 μg/mL for E. coli K88, and Gram-positive bacteria with a MIC of 5–5.5 μg/mL for Bacillus subtilis KCCM21366, and 12.5–13.5 μg/mL for Staphylococcus aureus ATCC 2592). Generally, our results indicate that this expression system successfully can produce active GST-fused antibacterial peptides with a large-scale industrial amount..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
|---|---|
| Enthalten in: |
International journal of peptide research and therapeutics - 28(2022), 2 vom: 09. Feb. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Taghizadeh, Saeed [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: | |
|---|---|
| Themen: |
Antibacterial activity |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2022 |
|---|
| doi: |
10.1007/s10989-022-10374-5 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2078003859 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2078003859 | ||
| 003 | DE-627 | ||
| 005 | 20230518142800.0 | ||
| 007 | tu | ||
| 008 | 221220s2022 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10989-022-10374-5 |2 doi | |
| 035 | |a (DE-627)OLC2078003859 | ||
| 035 | |a (DE-He213)s10989-022-10374-5-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 540 |q VZ |
| 084 | |a 35.76$jAminosäuren$jPeptide$jEiweiße$XBiochemie |2 bkl | ||
| 100 | 1 | |a Taghizadeh, Saeed |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Recombinant Expression and Antibacterial Properties of BmTXKS2 Venom Peptide in Fusion with GST |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer Nature B.V. 2022 | ||
| 520 | |a Abstract The increase in antibiotic-resistant bacteria cases is an immediate global health problem. Scorpion venom is an excellent candidate for new antimicrobial drug development due to its ability to prevent drug resistance with fewer side effects. BmTXKS2 is a venom peptide identified from a full-length cDNA clone from the venom gland of the Chinese scorpion, Buthus martensii Karsch. It is predicted to have 39 residues with three disulfide bridges and have similarity to hemolymph defensins. In this work, GST-His-BmTXKS2 plasmid was chemically synthesized, inserted into vector pGEX-4T-1, and expressed in Escherichia coli (E. coli). The glutathione-S-transferase (GST) fusion product of BmTXKS2 in a pure form was expressed in a sufficient amount to characterize its antibacterial activity which was assayed through the minimum inhibitory concentration (MIC) method and inhibition zone assay. GST-His-BmTXKS2 exhibited potent inhibitory effects on the four strains (Gram-negative bacteria with a MIC of 12.5–13 μg/mL for E. coli ATCC 25922, 18–19.5 μg/mL for E. coli K88, and Gram-positive bacteria with a MIC of 5–5.5 μg/mL for Bacillus subtilis KCCM21366, and 12.5–13.5 μg/mL for Staphylococcus aureus ATCC 2592). Generally, our results indicate that this expression system successfully can produce active GST-fused antibacterial peptides with a large-scale industrial amount. | ||
| 650 | 4 | |a BmTXKS2 | |
| 650 | 4 | |a Venom peptide | |
| 650 | 4 | |a Antibacterial activity | |
| 650 | 4 | |a GST fused expression | |
| 700 | 1 | |a Savardashtaki, Amir |4 aut | |
| 700 | 1 | |a Irajie, Cambyz |4 aut | |
| 700 | 1 | |a Rahbar, Mohammad Reza |4 aut | |
| 700 | 1 | |a Ghasemi, Younes |0 (orcid)0000-0003-4172-0672 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of peptide research and therapeutics |d Springer Netherlands, 2005 |g 28(2022), 2 vom: 09. Feb. |w (DE-627)490328148 |w (DE-600)2193110-0 |x 1573-3149 |7 nnns |
| 773 | 1 | 8 | |g volume:28 |g year:2022 |g number:2 |g day:09 |g month:02 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s10989-022-10374-5 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 936 | b | k | |a 35.76$jAminosäuren$jPeptide$jEiweiße$XBiochemie |q VZ |0 181571129 |0 (DE-625)181571129 |
| 951 | |a AR | ||
| 952 | |d 28 |j 2022 |e 2 |b 09 |c 02 | ||