Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line
Abstract Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing Pigm into C134S and showed significant differences in the panicle blast resistance. One $ F_{2} $ population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL qPBR10-1 on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore, qPBR10-1 was verified using the $ BC_{1} $$ F_{2} $ and $ BC_{1} $$ F_{3} $ population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that ORF1 and ORF2 encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the qPBR10-1 locus..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:41 |
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| Enthalten in: |
Molecular breeding - 41(2021), 12 vom: 30. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Yunyu [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
Candidate gene prediction |
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| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2021 |
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| doi: |
10.1007/s11032-021-01268-3 |
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| funding: |
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OLC2077555386 |
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| 520 | |a Abstract Rice blast is one of the most widespread and devastating diseases in rice production. Tremendous success has been achieved in the identification and characterization of genes and quantitative trait loci (QTLs) conferring seedling blast resistance, however, genetic studies on panicle blast resistance have lagged far behind. In this study, two advanced backcross inbred sister lines (MSJ13 and MSJ18) were obtained in the process of introducing Pigm into C134S and showed significant differences in the panicle blast resistance. One $ F_{2} $ population derived from the crossing MSJ13/MSJ18 was used to QTL mapping for panicle blast resistance using genotyping by sequencing (GBS) method. A total of seven QTLs were identified, including a major QTL qPBR10-1 on chromosome 10 that explains 24.21% of phenotypic variance with LOD scores of 6.62. Furthermore, qPBR10-1 was verified using the $ BC_{1} $$ F_{2} $ and $ BC_{1} $$ F_{3} $ population and narrowed to a 60.6-kb region with six candidate genes predicted, including two genes encoding exonuclease family protein, two genes encoding hypothetical protein, and two genes encoding transposon protein. The nucleotide variations and the expression patterns of the candidate genes were identified and analyzed between MSJ13 and MSJ18 through sequence comparison and RT-PCR approach, and results indicated that ORF1 and ORF2 encoding exonuclease family protein might be the causal candidate genes for panicle blast resistance in the qPBR10-1 locus. | ||
| 650 | 4 | |a Panicle blast resistance | |
| 650 | 4 | |a Genotyping by sequencing (GBS) | |
| 650 | 4 | |a Quantitative trait loci (QTLs) | |
| 650 | 4 | |a Fine mapping | |
| 650 | 4 | |a Candidate gene prediction | |
| 700 | 1 | |a Xiao, Ning |4 aut | |
| 700 | 1 | |a Li, Yuhong |4 aut | |
| 700 | 1 | |a Gao, Qiang |4 aut | |
| 700 | 1 | |a Ning, Yuese |4 aut | |
| 700 | 1 | |a Yu, Ling |4 aut | |
| 700 | 1 | |a Cai, Yue |4 aut | |
| 700 | 1 | |a Pan, Cunhong |4 aut | |
| 700 | 1 | |a Zhang, Xiaoxiang |4 aut | |
| 700 | 1 | |a Huang, Niansheng |4 aut | |
| 700 | 1 | |a Zhou, Changhai |4 aut | |
| 700 | 1 | |a Ji, Hongjuan |4 aut | |
| 700 | 1 | |a Liu, Jianju |4 aut | |
| 700 | 1 | |a Shi, Wei |4 aut | |
| 700 | 1 | |a Chen, Zichun |4 aut | |
| 700 | 1 | |a Liang, Chengzhi |4 aut | |
| 700 | 1 | |a Li, Aihong |4 aut | |
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