Effect of storage on plant biostimulant and bioactive properties of freeze-dried Chlorella vulgaris biomass
Abstract Microalgae are potential plant biostimulants and biocontrol agents. A major hurdle towards their commercialization is the production of large volumes of biomass at the correct time of year. Secondary metabolites are unstable and the “shelf-life” of bioactive microalgal biomass needs to be investigated. The aim of the study was to investigate the effects of storage conditions on freeze-dried microalgae to determine how long the biomass retained its growth promoting and bioactive properties under various temperature and light conditions. Chlorella vulgaris biomass was stored in the dark at − 70 °C, 10 °C, and 25 °C and in the light at 25 °C. Samples were tested every 3–4 months for 15 months. Storage time significantly influenced the rate of change in the bioactivity in the C. vulgaris biomass with storage temperature also having some effect. Rooting activity decreased in the mungbean rooting assay over time up to 12 months and then increased slightly. Antimicrobial activity increased against Staphylococcus aureus and Escherichia coli for up to 12 months and then declined. Antioxidant activity measured in the DPPH assay remained relatively stable for up to 12 months and then significantly decreased with longer storage. The change in bioactivity over time was attributed to the gradual breakdown of the rigid cell wall of C. vulgaris, thereby improving extraction efficiency but exposing the secondary metabolites to oxygen, thus quickening their degradation. Biomass produced for commercial purposes requires preliminary validation as the results of the present study showed that bioactive compounds are susceptible to degradation over time..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
|---|---|
| Enthalten in: |
Journal of applied phycology - 33(2021), 6 vom: 14. Sept., Seite 3797-3806 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Stirk, Wendy A. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: | |
|---|---|
| Themen: |
Antimicrobial activity |
| Anmerkungen: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2021 |
|---|
| doi: |
10.1007/s10811-021-02596-9 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2077484640 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2077484640 | ||
| 003 | DE-627 | ||
| 005 | 20230517011209.0 | ||
| 007 | tu | ||
| 008 | 221220s2021 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10811-021-02596-9 |2 doi | |
| 035 | |a (DE-627)OLC2077484640 | ||
| 035 | |a (DE-He213)s10811-021-02596-9-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 580 |a 570 |q VZ |
| 084 | |a 12 |a 14 |2 ssgn | ||
| 084 | |a BIODIV |q DE-30 |2 fid | ||
| 084 | |a 42.00 |2 bkl | ||
| 100 | 1 | |a Stirk, Wendy A. |e verfasserin |0 (orcid)0000-0003-4033-5641 |4 aut | |
| 245 | 1 | 0 | |a Effect of storage on plant biostimulant and bioactive properties of freeze-dried Chlorella vulgaris biomass |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © The Author(s), under exclusive licence to Springer Nature B.V. 2021 | ||
| 520 | |a Abstract Microalgae are potential plant biostimulants and biocontrol agents. A major hurdle towards their commercialization is the production of large volumes of biomass at the correct time of year. Secondary metabolites are unstable and the “shelf-life” of bioactive microalgal biomass needs to be investigated. The aim of the study was to investigate the effects of storage conditions on freeze-dried microalgae to determine how long the biomass retained its growth promoting and bioactive properties under various temperature and light conditions. Chlorella vulgaris biomass was stored in the dark at − 70 °C, 10 °C, and 25 °C and in the light at 25 °C. Samples were tested every 3–4 months for 15 months. Storage time significantly influenced the rate of change in the bioactivity in the C. vulgaris biomass with storage temperature also having some effect. Rooting activity decreased in the mungbean rooting assay over time up to 12 months and then increased slightly. Antimicrobial activity increased against Staphylococcus aureus and Escherichia coli for up to 12 months and then declined. Antioxidant activity measured in the DPPH assay remained relatively stable for up to 12 months and then significantly decreased with longer storage. The change in bioactivity over time was attributed to the gradual breakdown of the rigid cell wall of C. vulgaris, thereby improving extraction efficiency but exposing the secondary metabolites to oxygen, thus quickening their degradation. Biomass produced for commercial purposes requires preliminary validation as the results of the present study showed that bioactive compounds are susceptible to degradation over time. | ||
| 650 | 4 | |a Antimicrobial activity | |
| 650 | 4 | |a Antioxidant activity | |
| 650 | 4 | |a Light | |
| 650 | 4 | |a Rooting activity | |
| 650 | 4 | |a Storage time | |
| 650 | 4 | |a Temperature | |
| 700 | 1 | |a Bálint, Péter |4 aut | |
| 700 | 1 | |a Vambe, McMaster |0 (orcid)0000-0001-7452-7225 |4 aut | |
| 700 | 1 | |a Kulkarni, Manoj G. |0 (orcid)0000-0001-9656-4676 |4 aut | |
| 700 | 1 | |a van Staden, Johannes |0 (orcid)0000-0003-0515-1281 |4 aut | |
| 700 | 1 | |a Ördög, Vince |0 (orcid)0000-0001-7719-8885 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of applied phycology |d Springer Netherlands, 1989 |g 33(2021), 6 vom: 14. Sept., Seite 3797-3806 |w (DE-627)13077149X |w (DE-600)1002324-0 |w (DE-576)023036389 |x 0921-8971 |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2021 |g number:6 |g day:14 |g month:09 |g pages:3797-3806 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s10811-021-02596-9 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a SSG-OPC-GGO | ||
| 912 | |a GBV_ILN_118 | ||
| 912 | |a GBV_ILN_601 | ||
| 936 | b | k | |a 42.00 |q VZ |
| 951 | |a AR | ||
| 952 | |d 33 |j 2021 |e 6 |b 14 |c 09 |h 3797-3806 | ||