Occurrence of Extended-Spectrum Betalactamases (ESBL) in Dutch Hospitals
Summary The prevalence of ESBL was determined among isolates of Escherichia coli (n = 571) and Klebsiella spp. (n = 196) collected during a 1-week study period in 8 university and 3 large regional laboratories all over the Netherlands. 18 isolates were positive for at least one of the screening tests used, i.e., VITEK-ESBL, E-test ESBL and MIC ratio of ceftazidime/ceftazidime-clavulanic acid, cefotaxime/cefotaxime-clavulanic acid. In 5 of these 18 putative ESBLs no betalactamase production was detectable. A TEM type was found in three E. coli and two Klebsiella spp. An SHV type was present in five Klebsiella spp. In one E. coli and one Klebsiella pneumoniae both enzymes were present. In one Klebsiella oxytoca neither of the two enzymes was present. Using PCR for both ESBL TEM and ESBL SHV, an SHV ESBL was found in one E. coli and four Klebsiella isolates. The mutations at position 238 and 240 were already described. In one E. coli isolate a TEM ESBL was found with three mutations, at position 21, 164 and 265. These mutations were already described in other ESBLs but not in this combination suggesting a new TEM ESBL. The overall prevalence of ESBL producing E. coli and Klebsiella spp. was less than 1% (6 out of 767)..
Medienart: |
Artikel |
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Erscheinungsjahr: |
1999 |
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Erschienen: |
1999 |
Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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Enthalten in: |
Infection - 27(1999), 6 vom: Dez., Seite 348-354 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Stobberingh, E.E. [VerfasserIn] |
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Links: |
Volltext [lizenzpflichtig] |
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Anmerkungen: |
© Urban & Vogel Medien und Medizin Verlagsgesellschaft 1999 |
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doi: |
10.1007/s150100050041 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
OLC2051832501 |
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245 | 1 | 0 | |a Occurrence of Extended-Spectrum Betalactamases (ESBL) in Dutch Hospitals |
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520 | |a Summary The prevalence of ESBL was determined among isolates of Escherichia coli (n = 571) and Klebsiella spp. (n = 196) collected during a 1-week study period in 8 university and 3 large regional laboratories all over the Netherlands. 18 isolates were positive for at least one of the screening tests used, i.e., VITEK-ESBL, E-test ESBL and MIC ratio of ceftazidime/ceftazidime-clavulanic acid, cefotaxime/cefotaxime-clavulanic acid. In 5 of these 18 putative ESBLs no betalactamase production was detectable. A TEM type was found in three E. coli and two Klebsiella spp. An SHV type was present in five Klebsiella spp. In one E. coli and one Klebsiella pneumoniae both enzymes were present. In one Klebsiella oxytoca neither of the two enzymes was present. Using PCR for both ESBL TEM and ESBL SHV, an SHV ESBL was found in one E. coli and four Klebsiella isolates. The mutations at position 238 and 240 were already described. In one E. coli isolate a TEM ESBL was found with three mutations, at position 21, 164 and 265. These mutations were already described in other ESBLs but not in this combination suggesting a new TEM ESBL. The overall prevalence of ESBL producing E. coli and Klebsiella spp. was less than 1% (6 out of 767). | ||
700 | 1 | |a Arends, J. |4 aut | |
700 | 1 | |a Hoggkamp-Korstanje, J.A.A. |4 aut | |
700 | 1 | |a Goessens, W.H.F. |4 aut | |
700 | 1 | |a Visser, M.R. |4 aut | |
700 | 1 | |a Buiting, A.G.M. |4 aut | |
700 | 1 | |a Debets-Ossenkopp, Y.J. |4 aut | |
700 | 1 | |a van Ketel, R.J. |4 aut | |
700 | 1 | |a van Ogtrop, M.L. |4 aut | |
700 | 1 | |a Sabbe, L.J.M. |4 aut | |
700 | 1 | |a Voorn, G.P. |4 aut | |
700 | 1 | |a Winter, H.L.J. |4 aut | |
700 | 1 | |a van Zeijl, J.H. |4 aut | |
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