Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer
Abstract MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer..
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2017 |
---|---|
Erschienen: |
2017 |
Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
---|---|
Enthalten in: |
Frontiers of medicine - 11(2017), 2 vom: 04. Mai, Seite 214-222 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhou, Bo [VerfasserIn] |
---|
Links: |
Volltext [lizenzpflichtig] |
---|
Themen: |
---|
Anmerkungen: |
© Higher Education Press and Springer-Verlag Berlin Heidelberg 2017 |
---|
doi: |
10.1007/s11684-017-0518-7 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
OLC205129142X |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | OLC205129142X | ||
003 | DE-627 | ||
005 | 20240404064426.0 | ||
007 | tu | ||
008 | 200819s2017 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1007/s11684-017-0518-7 |2 doi | |
035 | |a (DE-627)OLC205129142X | ||
035 | |a (DE-He213)s11684-017-0518-7-p | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 610 |q VZ |
100 | 1 | |a Zhou, Bo |e verfasserin |4 aut | |
245 | 1 | 0 | |a Overexpressed miR-9 promotes tumor metastasis via targeting E-cadherin in serous ovarian cancer |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a © Higher Education Press and Springer-Verlag Berlin Heidelberg 2017 | ||
520 | |a Abstract MicroRNAs (miRNAs) play critical roles in the development and progression in various cancers. Dysfunctional miR-9 expression remains ambiguous, and no consensus on the metastatic progression of ovarian cancer has been reached. In this study, results from the bioinformatics analysis show that the 3′-UTR of the E-cadherin mRNA was directly regulated by miR-9. Luciferase reporter assay results confirmed that miR-9 could directly target this 3′-UTR. miR-9 and E-cadherin expression in ovarian cancer tissue was quantified by qRT-PCR. Migration and invasion were detected by wound healing and Transwell system assay in SKOV3 and A2780. qRT-PCR and Western blot were performed to detect the epithelial‒mesenchymal transition-associated mRNA and proteins. Immunofluorescence technique was used to analyze the expression and subcellular localization of E-cadherin, N-cadherin, and vimentin. The results showed that miR-9 was frequently upregulated in metastatic serous ovarian cancer tissue compared with paired primary ones. Upregulation of miR-9 could downregulate the expression of E-cadherin but upregulate the expression of mesenchymal markers (N-cadherin and vimentin). Overexpression of miR-9 could promote the cell migration and invasion in ovarian cancer, and these processes could be effectively inhibited via miR-9 inhibitor. Thus, our study demonstrates that miR-9 may promote ovarian cancer metastasis via targeting E-cadherin and a novel potential therapeutic approach to control metastasis of ovarian cancer. | ||
650 | 4 | |a ovarian cancer | |
650 | 4 | |a metastasis | |
650 | 4 | |a miR-9 | |
650 | 4 | |a E-cadherin | |
700 | 1 | |a Xu, Hongbin |4 aut | |
700 | 1 | |a Xia, Meng |4 aut | |
700 | 1 | |a Sun, Chaoyang |4 aut | |
700 | 1 | |a Li, Na |4 aut | |
700 | 1 | |a Guo, Ensong |4 aut | |
700 | 1 | |a Guo, Lili |4 aut | |
700 | 1 | |a Shan, Wanying |4 aut | |
700 | 1 | |a Lu, Hao |4 aut | |
700 | 1 | |a Wu, Yifan |4 aut | |
700 | 1 | |a Li, Yuan |4 aut | |
700 | 1 | |a Yang, Degui |4 aut | |
700 | 1 | |a Weng, Danhui |4 aut | |
700 | 1 | |a Meng, Li |4 aut | |
700 | 1 | |a Hu, Junbo |4 aut | |
700 | 1 | |a Ma, Ding |4 aut | |
700 | 1 | |a Chen, Gang |4 aut | |
700 | 1 | |a Li, Kezhen |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Frontiers of medicine |d Higher Education Press, 2011 |g 11(2017), 2 vom: 04. Mai, Seite 214-222 |w (DE-627)671806890 |w (DE-600)2636271-5 |w (DE-576)352909021 |x 2095-0217 |7 nnns |
773 | 1 | 8 | |g volume:11 |g year:2017 |g number:2 |g day:04 |g month:05 |g pages:214-222 |
856 | 4 | 1 | |u https://doi.org/10.1007/s11684-017-0518-7 |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
912 | |a SSG-OLC-PHA | ||
912 | |a GBV_ILN_2018 | ||
951 | |a AR | ||
952 | |d 11 |j 2017 |e 2 |b 04 |c 05 |h 214-222 |