Characterisation and expression of a novel holotype crystal protein gene, cry56Aa1, from Bacillus thuringiensis strain Ywc2-8
Abstract Bacillus thuringiensis isolate Ywc2-8, from soil in Sichuan Basin in western China, contains a spherical crystal harbouring two insecticidal crystal proteins with masses of 70 kDa and 130 kDa. A novel cry-type gene, encoding a 664 amino acid protein with 34% homology to cry29Aa1, was found and cloned from this strain. This gene belongs to a novel holotype cry and is designated as cry56Aa1. It was expressed in E. coli. Insecticidal activity assays showed that recombinant Cry56Aa1 was toxic to both Dipteran (Aedes aegypti) and Lepidopteran (Plutella xylostella and Helicoverpa armigera) pests. Cloning of this gene may help to overcome the increasing resistance of pests to currently used insecticides..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Biotechnology letters - 32(2009), 2 vom: 17. Okt., Seite 283-288 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhu, J. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
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| Anmerkungen: |
© Springer Science+Business Media B.V. 2009 |
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| doi: |
10.1007/s10529-009-0147-6 |
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| PPN (Katalog-ID): |
OLC2050317549 |
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| 245 | 1 | 0 | |a Characterisation and expression of a novel holotype crystal protein gene, cry56Aa1, from Bacillus thuringiensis strain Ywc2-8 |
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| 520 | |a Abstract Bacillus thuringiensis isolate Ywc2-8, from soil in Sichuan Basin in western China, contains a spherical crystal harbouring two insecticidal crystal proteins with masses of 70 kDa and 130 kDa. A novel cry-type gene, encoding a 664 amino acid protein with 34% homology to cry29Aa1, was found and cloned from this strain. This gene belongs to a novel holotype cry and is designated as cry56Aa1. It was expressed in E. coli. Insecticidal activity assays showed that recombinant Cry56Aa1 was toxic to both Dipteran (Aedes aegypti) and Lepidopteran (Plutella xylostella and Helicoverpa armigera) pests. Cloning of this gene may help to overcome the increasing resistance of pests to currently used insecticides. | ||
| 650 | 4 | |a Characterisation | |
| 650 | 4 | |a Expression | |
| 650 | 4 | |a Insecticidal activity | |
| 700 | 1 | |a Zheng, A. P. |4 aut | |
| 700 | 1 | |a Tan, F. R. |4 aut | |
| 700 | 1 | |a Wang, S. Q. |4 aut | |
| 700 | 1 | |a Deng, Q. M. |4 aut | |
| 700 | 1 | |a Li, S. C. |4 aut | |
| 700 | 1 | |a Wang, L. X. |4 aut | |
| 700 | 1 | |a Li, P. |4 aut | |
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