Pleural Mesothelial Cells Promote Expansion of IL-17–Producing $ CD8^{+} $ T Cells in Tuberculous Pleural Effusion
Abstract IL-17–producing $ CD8^{+} $ T lymphocytes (Tc17 cells) have recently been detected in many cancers and autoimmune diseases. However, the possible implication of Tc17 cells in tuberculous pleural effusion remains unclarified. In this study, distribution and phenotypic features of Tc17 cells in both tuberculous pleural effusion (TPE) and peripheral blood from patients with tuberculosis were determined. The effects of proinflammatory cytokines and local accessory cells (pleural mesothelial cells) on Tc17 cell expansion were also explored. We found that TPE contained more Tc17 cells than the blood. Compared with IFN-γ–producing $ CD8^{+} $ T cells, Tc17 cells displayed higher expression of chemokine receptors (CCRs) and lower expression of cytotoxic molecules. In particularly, Tc17 cells in TPE exhibited high expression levels of CCR6, which could migrate in response to CCL20. Furthermore, IL-1β, IL-6, IL-23, or their various combinations could promote Tc17 cell expansion from $ CD8^{+} $ T cells, whereas the proliferative response of Tc17 cells to above cytokines was lower than that of Th17 cells. Pleural mesothelial cells (PMCs) were able to stimulate Tc17 cell expansion via cell contact in an IL-1β/IL-6/IL-23 independent fashion. Thus this study demonstrates that Tc17 cells marks a subset of non-cytotoxic, $ CCR6^{+} $ $ CD8^{+} $ T lymphocytes with low proliferative capacity. The overrepresentation of Tc17 cells in TPE may be due to Tc17 cell expansion stimulated by pleural proinflammatory cytokines and to recruitment of Tc17 cells from peripheral blood. Additionally, PMCs may promote the production of IL-17 by $ CD8^{+} $ T cells at sites of TPE via cell–cell interactions..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2013 |
|---|---|
| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
|---|---|
| Enthalten in: |
Journal of clinical immunology - 33(2013), 4 vom: 09. Jan., Seite 775-787 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, X. [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| BKL: | |
|---|---|
| Themen: |
Antigen-presenting cells |
| Anmerkungen: |
© Springer Science+Business Media New York 2013 |
|---|
| doi: |
10.1007/s10875-012-9860-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2049637047 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2049637047 | ||
| 003 | DE-627 | ||
| 005 | 20230503134557.0 | ||
| 007 | tu | ||
| 008 | 200819s2013 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10875-012-9860-3 |2 doi | |
| 035 | |a (DE-627)OLC2049637047 | ||
| 035 | |a (DE-He213)s10875-012-9860-3-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 610 |q VZ |
| 084 | |a 44.00 |2 bkl | ||
| 100 | 1 | |a Li, X. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Pleural Mesothelial Cells Promote Expansion of IL-17–Producing $ CD8^{+} $ T Cells in Tuberculous Pleural Effusion |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Springer Science+Business Media New York 2013 | ||
| 520 | |a Abstract IL-17–producing $ CD8^{+} $ T lymphocytes (Tc17 cells) have recently been detected in many cancers and autoimmune diseases. However, the possible implication of Tc17 cells in tuberculous pleural effusion remains unclarified. In this study, distribution and phenotypic features of Tc17 cells in both tuberculous pleural effusion (TPE) and peripheral blood from patients with tuberculosis were determined. The effects of proinflammatory cytokines and local accessory cells (pleural mesothelial cells) on Tc17 cell expansion were also explored. We found that TPE contained more Tc17 cells than the blood. Compared with IFN-γ–producing $ CD8^{+} $ T cells, Tc17 cells displayed higher expression of chemokine receptors (CCRs) and lower expression of cytotoxic molecules. In particularly, Tc17 cells in TPE exhibited high expression levels of CCR6, which could migrate in response to CCL20. Furthermore, IL-1β, IL-6, IL-23, or their various combinations could promote Tc17 cell expansion from $ CD8^{+} $ T cells, whereas the proliferative response of Tc17 cells to above cytokines was lower than that of Th17 cells. Pleural mesothelial cells (PMCs) were able to stimulate Tc17 cell expansion via cell contact in an IL-1β/IL-6/IL-23 independent fashion. Thus this study demonstrates that Tc17 cells marks a subset of non-cytotoxic, $ CCR6^{+} $ $ CD8^{+} $ T lymphocytes with low proliferative capacity. The overrepresentation of Tc17 cells in TPE may be due to Tc17 cell expansion stimulated by pleural proinflammatory cytokines and to recruitment of Tc17 cells from peripheral blood. Additionally, PMCs may promote the production of IL-17 by $ CD8^{+} $ T cells at sites of TPE via cell–cell interactions. | ||
| 650 | 4 | |a Antigen-presenting cells | |
| 650 | 4 | |a pleural mesothelial cells | |
| 650 | 4 | |a Tc17 cells | |
| 650 | 4 | |a tuberculosis | |
| 700 | 1 | |a Zhou, Q. |4 aut | |
| 700 | 1 | |a Yang, W. B. |4 aut | |
| 700 | 1 | |a Xiong, X. Z. |4 aut | |
| 700 | 1 | |a Du, R. H. |4 aut | |
| 700 | 1 | |a Zhang, J. C. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of clinical immunology |d Springer US, 1981 |g 33(2013), 4 vom: 09. Jan., Seite 775-787 |w (DE-627)168589001 |w (DE-600)779361-3 |w (DE-576)016104072 |x 0271-9142 |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2013 |g number:4 |g day:09 |g month:01 |g pages:775-787 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s10875-012-9860-3 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4219 | ||
| 936 | b | k | |a 44.00 |q VZ |
| 951 | |a AR | ||
| 952 | |d 33 |j 2013 |e 4 |b 09 |c 01 |h 775-787 | ||