siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells

Abstract The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS..

Medienart:

Artikel

Erscheinungsjahr:

2008

Erschienen:

2008

Enthalten in:

Zur Gesamtaufnahme - volume:27

Enthalten in:

European journal of clinical microbiology & infectious diseases - 27(2008), 8 vom: 01. Mai, Seite 709-715

Sprache:

Englisch

Beteiligte Personen:

Lu, C.-Y. [VerfasserIn]
Huang, H.-Y. [VerfasserIn]
Yang, T.-H. [VerfasserIn]
Chang, L.-Y. [VerfasserIn]
Lee, C.-Y. [VerfasserIn]
Huang, L.-M. [VerfasserIn]

Links:

Volltext [lizenzpflichtig]

Themen:

Quantitative Reverse Transcription Polymerase Chain Reaction
Reverse Transcription Polymerase Chain Reaction
Severe Acute Respiratory Syndrome
Severe Acute Respiratory Syndrome Patient

RVK:

RVK Klassifikation

Anmerkungen:

© Springer-Verlag 2008

doi:

10.1007/s10096-008-0495-5

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

OLC2045873536

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