The Development of a Sheathless Interface for Capillary Electrophoresis Electrospray Ionization Mass Spectrometry Using a Cellulose Acetate Cast Capillary
Abstract The fabrication of a novel sheathless interface for capillary electrophoresis–electrospray–mass spectrometry (CE–ESI–MS) is described. A programmable $ CO_{2} $ laser was used to ablate small channels in the walls of a polyimide capillary near the terminus. Subsequent exposure of the channel region to a cellulose acetate solution followed by drying resulted in the formation of an electrically conductive semi-permeable membrane. Application of an appropriate voltage to the reservoir resulted in the simultaneous establishment of an electrical connection for CE and ESI. Interface viability was demonstrated by conducting a CE separation of a peptide mixture, with detection accomplished via positive ion mode ESI–MS. For the peptide Val-Tyr-Val, a limit of detection of 0.1 femtomole (S/N 3) was achieved using single reaction monitoring. Attributes of the interface include structural robustness, ease of fabrication, minimal interface dead volume, and the ability to alter post-separation analyte ionization status by use of appropriate buffers in the interface reservoir..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:80 |
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| Enthalten in: |
Chromatographia - 80(2017), 7 vom: 17. Mai, Seite 1061-1067 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Johnson, Ryan T. [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
CE–ESI–MS interface |
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| RVK: |
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| Anmerkungen: |
© Springer-Verlag Berlin Heidelberg 2017 |
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| doi: |
10.1007/s10337-017-3326-y |
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| funding: |
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| PPN (Katalog-ID): |
OLC2044366029 |
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| 520 | |a Abstract The fabrication of a novel sheathless interface for capillary electrophoresis–electrospray–mass spectrometry (CE–ESI–MS) is described. A programmable $ CO_{2} $ laser was used to ablate small channels in the walls of a polyimide capillary near the terminus. Subsequent exposure of the channel region to a cellulose acetate solution followed by drying resulted in the formation of an electrically conductive semi-permeable membrane. Application of an appropriate voltage to the reservoir resulted in the simultaneous establishment of an electrical connection for CE and ESI. Interface viability was demonstrated by conducting a CE separation of a peptide mixture, with detection accomplished via positive ion mode ESI–MS. For the peptide Val-Tyr-Val, a limit of detection of 0.1 femtomole (S/N 3) was achieved using single reaction monitoring. Attributes of the interface include structural robustness, ease of fabrication, minimal interface dead volume, and the ability to alter post-separation analyte ionization status by use of appropriate buffers in the interface reservoir. | ||
| 650 | 4 | |a CE–ESI–MS interface | |
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| 650 | 4 | |a Standards | |
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| 700 | 1 | |a Stobaugh, John F. |4 aut | |
| 700 | 1 | |a Lunte, Craig E. |4 aut | |
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