Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India
Abstract In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction–restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing..
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2012 |
---|---|
Erschienen: |
2012 |
Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
---|---|
Enthalten in: |
Tropical animal health and production - 44(2012), 7 vom: 10. Mai, Seite 1791-1795 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Sumi, Vikshe [VerfasserIn] |
---|
Links: |
Volltext [lizenzpflichtig] |
---|
Themen: |
4/91 strain |
---|
Anmerkungen: |
© Springer Science+Business Media B.V. 2012 |
---|
doi: |
10.1007/s11250-012-0140-2 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
OLC2042170690 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | OLC2042170690 | ||
003 | DE-627 | ||
005 | 20230516220615.0 | ||
007 | tu | ||
008 | 200819s2012 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1007/s11250-012-0140-2 |2 doi | |
035 | |a (DE-627)OLC2042170690 | ||
035 | |a (DE-He213)s11250-012-0140-2-p | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 630 |q VZ |
100 | 1 | |a Sumi, Vikshe |e verfasserin |4 aut | |
245 | 1 | 0 | |a Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India |
264 | 1 | |c 2012 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a © Springer Science+Business Media B.V. 2012 | ||
520 | |a Abstract In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction–restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing. | ||
650 | 4 | |a Infectious bronchitis | |
650 | 4 | |a 4/91 strain | |
650 | 4 | |a Molecular characterization | |
650 | 4 | |a S1 gene | |
650 | 4 | |a India | |
700 | 1 | |a Singh, Shambhu Dayal |4 aut | |
700 | 1 | |a Dhama, Kuldeep |4 aut | |
700 | 1 | |a Gowthaman, Vasudevan |4 aut | |
700 | 1 | |a Barathidasan, Rajamani |4 aut | |
700 | 1 | |a Sukumar, Kuppannan |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Tropical animal health and production |d Springer Netherlands, 1969 |g 44(2012), 7 vom: 10. Mai, Seite 1791-1795 |w (DE-627)13039730X |w (DE-600)603363-5 |w (DE-576)015900258 |x 0049-4747 |7 nnns |
773 | 1 | 8 | |g volume:44 |g year:2012 |g number:7 |g day:10 |g month:05 |g pages:1791-1795 |
856 | 4 | 1 | |u https://doi.org/10.1007/s11250-012-0140-2 |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
912 | |a SSG-OLC-PHA | ||
912 | |a SSG-OLC-DE-84 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_4012 | ||
951 | |a AR | ||
952 | |d 44 |j 2012 |e 7 |b 10 |c 05 |h 1791-1795 |