Differential responses to isoprenoid, N6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus
Abstract Eriocephalus africanus is a medicinal and aromatic plant species that is part of South Africa’s remarkable diversity. As a result of illegal and over-harvesting, most plant communities have become unsustainable and as such, effective and efficient conservation strategies have to be implemented. In the present study, an isoprenoid cytokinin (CK): isopentenyladenine (iP) and four aromatic CKs namely benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR) and 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine (mTTHP) at 1, 5 or 10 µM were evaluated for in vitro plant regeneration in E. africanus. Different concentrations of indole-3-butyric acid (IBA) were also evaluated for shoot and root organogenesis. The highest number of shoots was produced by mT (1 and 5 µM) treatment, longest shoots were stimulated by iP (1 µM) and the highest fresh mass was obtained in BA (5 and 10 µM), mT (5 and 10 µM) and mTTHP (5 µM)-treated plants. During acclimatization, all the in vitro plants obtained from the lowest concentration of CKs survived and 1 µM iP regenerants recorded a 100% survival rate. For the rooting experiment, more vigorous adventitious roots were observed in the 1 µM IBA treatment. All IBA treatments had 100% survival rate after 6 weeks of acclimatization. Overall, the concentration and type of plant growth regulators had a remarkable influence on the growth and development of in vitro-derived E. africanus..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:82 |
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| Enthalten in: |
Plant growth regulation - 82(2017), 1 vom: 09. Feb., Seite 103-110 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Madzikane-Mlungwana, Olwethu [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| Themen: |
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| Anmerkungen: |
© Springer Science+Business Media Dordrecht 2017 |
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| doi: |
10.1007/s10725-016-0242-3 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2039361898 |
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| 520 | |a Abstract Eriocephalus africanus is a medicinal and aromatic plant species that is part of South Africa’s remarkable diversity. As a result of illegal and over-harvesting, most plant communities have become unsustainable and as such, effective and efficient conservation strategies have to be implemented. In the present study, an isoprenoid cytokinin (CK): isopentenyladenine (iP) and four aromatic CKs namely benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR) and 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine (mTTHP) at 1, 5 or 10 µM were evaluated for in vitro plant regeneration in E. africanus. Different concentrations of indole-3-butyric acid (IBA) were also evaluated for shoot and root organogenesis. The highest number of shoots was produced by mT (1 and 5 µM) treatment, longest shoots were stimulated by iP (1 µM) and the highest fresh mass was obtained in BA (5 and 10 µM), mT (5 and 10 µM) and mTTHP (5 µM)-treated plants. During acclimatization, all the in vitro plants obtained from the lowest concentration of CKs survived and 1 µM iP regenerants recorded a 100% survival rate. For the rooting experiment, more vigorous adventitious roots were observed in the 1 µM IBA treatment. All IBA treatments had 100% survival rate after 6 weeks of acclimatization. Overall, the concentration and type of plant growth regulators had a remarkable influence on the growth and development of in vitro-derived E. africanus. | ||
| 650 | 4 | |a Auxins | |
| 650 | 4 | |a Cytokinins | |
| 650 | 4 | |a Flavonoids | |
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| 700 | 1 | |a Moyo, Mack |4 aut | |
| 700 | 1 | |a Aremu, Adeyemi O. |4 aut | |
| 700 | 1 | |a Plíhalová, Lucie |4 aut | |
| 700 | 1 | |a Doležal, Karel |4 aut | |
| 700 | 1 | |a Van Staden, Johannes |4 aut | |
| 700 | 1 | |a Finnie, Jeffrey F. |4 aut | |
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