Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium
Purpose In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C. Methods Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min. Results Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21. Conclusion This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
Journal of assisted reproduction and genetics - 26(2009), 8 vom: Aug., Seite 467-473 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Datta, Uttam [VerfasserIn] |
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| Links: |
Volltext [lizenzpflichtig] |
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| BKL: | |
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| Themen: |
Coenzyme Q10 |
| Anmerkungen: |
© Springer Science+Business Media, LLC 2009 |
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| doi: |
10.1007/s10815-009-9344-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC2026194181 |
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| 245 | 1 | 0 | |a Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10°C with electrolyte free medium |
| 264 | 1 | |c 2009 | |
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| 520 | |a Purpose In-situ preservation of cauda epididymal spermatozoa at -10°C with electrolyte free media for obtaining maximum functional gametes than preservation at 5°C. Methods Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 — glycerol and soybean lecithin — Coenzyme Q10— glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5°C, wrapped with aluminium foil and freezed at — 10°C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5°C in a water bath for 5 min. Results Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 ± 0.62; 45.2 ± 0.82 and 41.6 ± 0.79 percent respectively on day 21. Conclusion This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter. | ||
| 650 | 4 | |a Coenzyme Q10 | |
| 650 | 4 | |a Electrolyte free medium | |
| 650 | 4 | |a Epididymal spermatozoa | |
| 650 | 4 | |a Freezing | |
| 650 | 4 | |a Hypo-osmotic swelling test | |
| 700 | 1 | |a Chandra Sekar, M. |4 aut | |
| 700 | 1 | |a Hembram, Manik Lal |4 aut | |
| 700 | 1 | |a Dasgupta, Raju |4 aut | |
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