Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen
Abstract Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:475 |
|---|---|
| Enthalten in: |
Virchows Archiv - 475(2019), 2 vom: 01. Juli, Seite 191-199 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
van Seijen, Maartje [VerfasserIn] |
|---|
| Links: |
Volltext [lizenzpflichtig] |
|---|
| Themen: |
Fixation |
|---|
| RVK: |
|---|
| Anmerkungen: |
© The Author(s) 2019 |
|---|
| doi: |
10.1007/s00428-019-02595-9 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC2025388829 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2025388829 | ||
| 003 | DE-627 | ||
| 005 | 20230519071430.0 | ||
| 007 | tu | ||
| 008 | 200819s2019 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00428-019-02595-9 |2 doi | |
| 035 | |a (DE-627)OLC2025388829 | ||
| 035 | |a (DE-He213)s00428-019-02595-9-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 610 |q VZ |
| 082 | 0 | 4 | |a 610 |q VZ |
| 084 | |a XA 27000 |q VZ |2 rvk | ||
| 100 | 1 | |a van Seijen, Maartje |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © The Author(s) 2019 | ||
| 520 | |a Abstract Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen. | ||
| 650 | 4 | |a Pre-analytical | |
| 650 | 4 | |a Fixation | |
| 650 | 4 | |a Immunohistochemistry | |
| 650 | 4 | |a Lung adenocarcinoma | |
| 700 | 1 | |a Brcic, Luka |4 aut | |
| 700 | 1 | |a Gonzales, Atilio Navarro |4 aut | |
| 700 | 1 | |a Sansano, Irene |4 aut | |
| 700 | 1 | |a Bendek, Matyas |4 aut | |
| 700 | 1 | |a Brcic, Iva |4 aut | |
| 700 | 1 | |a Lissenberg-Witte, Birgit |4 aut | |
| 700 | 1 | |a Korkmaz, H. Ibrahim |4 aut | |
| 700 | 1 | |a Geiger, Thomas |4 aut | |
| 700 | 1 | |a Kammler, Rosita |4 aut | |
| 700 | 1 | |a Stahel, Rolf |4 aut | |
| 700 | 1 | |a Thunnissen, Erik |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Virchows Archiv |d Springer Berlin Heidelberg, 1994 |g 475(2019), 2 vom: 01. Juli, Seite 191-199 |w (DE-627)18219146X |w (DE-600)1184867-4 |w (DE-576)038887061 |x 0945-6317 |7 nnns |
| 773 | 1 | 8 | |g volume:475 |g year:2019 |g number:2 |g day:01 |g month:07 |g pages:191-199 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s00428-019-02595-9 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_267 | ||
| 912 | |a GBV_ILN_711 | ||
| 912 | |a GBV_ILN_2018 | ||
| 912 | |a GBV_ILN_2414 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4219 | ||
| 912 | |a GBV_ILN_4277 | ||
| 936 | r | v | |a XA 27000 |
| 951 | |a AR | ||
| 952 | |d 475 |j 2019 |e 2 |b 01 |c 07 |h 191-199 | ||