Chlorella vulgaris Induces Apoptosis of Human Non-Small Cell Lung Carcinoma (NSCLC) Cells
Objective: Chlorella vulgaris (C. vulgaris), a unicellular green microalga, has been widely used as a food supplement and reported to have antioxidant and anticancer properties. The current study was designed to assess the cytotoxic, apoptotic, and DNA-damaging effects of C. vulgaris growth factor (CGF), hot water C. vulgaris extracts, inlung tumor A549 and NCI-H460 cell lines. Methods: A549 cells, NCI-H460 cells, and normal human fibroblasts were treated with CGF at various concentrations (0-300 ?g/ml) for 24 hr. The comet assay and ?H2AX assay showed DNA damage in A549 and NCI-H460 cells upon CGF exposure. Evaluation of apoptosis by the TUNEL assay and DNA fragmentation analysis by agarose gel electrophoresis showed that CGF induced apoptosis in A549 and NCI-H460 cells. Results: Chlorella vulgaris hot water extract induced apoptosis and DNA damage in human lung carcinoma cells. Conclusion: CGF can thus be considered a potential cytotoxic or genotoxic drug for treatment of lung carcinoma..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Medicinal chemistry - 13(2017), 6, Seite 560-568 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhi-Dong Zhang [VerfasserIn] |
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OLC1998517284 |
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| 520 | |a Objective: Chlorella vulgaris (C. vulgaris), a unicellular green microalga, has been widely used as a food supplement and reported to have antioxidant and anticancer properties. The current study was designed to assess the cytotoxic, apoptotic, and DNA-damaging effects of C. vulgaris growth factor (CGF), hot water C. vulgaris extracts, inlung tumor A549 and NCI-H460 cell lines. Methods: A549 cells, NCI-H460 cells, and normal human fibroblasts were treated with CGF at various concentrations (0-300 ?g/ml) for 24 hr. The comet assay and ?H2AX assay showed DNA damage in A549 and NCI-H460 cells upon CGF exposure. Evaluation of apoptosis by the TUNEL assay and DNA fragmentation analysis by agarose gel electrophoresis showed that CGF induced apoptosis in A549 and NCI-H460 cells. Results: Chlorella vulgaris hot water extract induced apoptosis and DNA damage in human lung carcinoma cells. Conclusion: CGF can thus be considered a potential cytotoxic or genotoxic drug for treatment of lung carcinoma. | ||
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