Immune responses in mice vaccinated with a DNA vaccine expressing serine protease-like protein from the new-born larval stage of Trichinella spiralis
SUMMARY Trichinella spiralis is a parasitic helminth that can infect almost all mammals, including humans. Trichinella spiralis infection elicits a typical type 2 immune responses, while suppresses type 1 immune responses, which is in favour of their parasitism. DNA vaccines have been shown to be capable of eliciting balanced CD4+ and CD8+ T cell responses as well as humoral immune responses in small-animal models, which will be advantage to induce protective immune response against helminth infection. In this study, serine protease (Ts-NBLsp) was encoded by a cDNA fragment of new-born T. spiralis larvae, and was inserted after CMV promoter to construct a DNA vaccine [pcDNA3·1(+)-Ts-NBLsp]. Ts-NBLsp expression was demonstrated by immunofluorescence. Sera samples were obtained from vaccinated mice, and they showed strong anti-Ts-NBLsp-specific IgG response. Mice immunized with the pcDNA3·1(+)-Ts-NBLsp DNA vaccine showed a 77·93% reduction in muscle larvae (ML) following challenge with T. spiralis ML. Our results demonstrate that the vaccination with pcDNA3·1(+)-Ts-NBLsp plasmid promoted the balance of type 1 and 2 immune responses and produced a significant protection against T. spiralis infection in mice..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:144 |
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| Enthalten in: |
Parasitology - 144(2017), 6, Seite 1-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
XU, JING [VerfasserIn] |
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| Links: |
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| RVK: |
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| doi: |
10.1017/S0031182016002493 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC1994145048 |
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| 100 | 1 | |a XU, JING |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Immune responses in mice vaccinated with a DNA vaccine expressing serine protease-like protein from the new-born larval stage of Trichinella spiralis |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a SUMMARY Trichinella spiralis is a parasitic helminth that can infect almost all mammals, including humans. Trichinella spiralis infection elicits a typical type 2 immune responses, while suppresses type 1 immune responses, which is in favour of their parasitism. DNA vaccines have been shown to be capable of eliciting balanced CD4+ and CD8+ T cell responses as well as humoral immune responses in small-animal models, which will be advantage to induce protective immune response against helminth infection. In this study, serine protease (Ts-NBLsp) was encoded by a cDNA fragment of new-born T. spiralis larvae, and was inserted after CMV promoter to construct a DNA vaccine [pcDNA3·1(+)-Ts-NBLsp]. Ts-NBLsp expression was demonstrated by immunofluorescence. Sera samples were obtained from vaccinated mice, and they showed strong anti-Ts-NBLsp-specific IgG response. Mice immunized with the pcDNA3·1(+)-Ts-NBLsp DNA vaccine showed a 77·93% reduction in muscle larvae (ML) following challenge with T. spiralis ML. Our results demonstrate that the vaccination with pcDNA3·1(+)-Ts-NBLsp plasmid promoted the balance of type 1 and 2 immune responses and produced a significant protection against T. spiralis infection in mice. | ||
| 650 | 4 | |a Parasites | |
| 650 | 4 | |a Animals | |
| 650 | 4 | |a Immune system | |
| 650 | 4 | |a Animal models | |
| 650 | 4 | |a Parasitism | |
| 650 | 4 | |a Public health | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a CD4 antigen | |
| 650 | 4 | |a Protease | |
| 650 | 4 | |a Antigens | |
| 650 | 4 | |a Infectious diseases | |
| 650 | 4 | |a DNA vaccines | |
| 650 | 4 | |a Rabies | |
| 650 | 4 | |a Deoxyribonucleic acid | |
| 650 | 4 | |a Immune response (humoral) | |
| 650 | 4 | |a Serine | |
| 650 | 4 | |a Infections | |
| 650 | 4 | |a Coding | |
| 650 | 4 | |a Immune systems | |
| 650 | 4 | |a Serine proteinase | |
| 650 | 4 | |a Laboratories | |
| 650 | 4 | |a Larvae | |
| 650 | 4 | |a Cloning | |
| 650 | 4 | |a Deoxyribonucleic acid--DNA | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a Tropical diseases | |
| 650 | 4 | |a Immunofluorescence | |
| 650 | 4 | |a CD8 antigen | |
| 650 | 4 | |a Vaccination | |
| 650 | 4 | |a Immunization | |
| 650 | 4 | |a Zoonoses | |
| 650 | 4 | |a Parasitology | |
| 650 | 4 | |a Epidemiology | |
| 650 | 4 | |a DNA | |
| 650 | 4 | |a Immunoglobulin G | |
| 650 | 4 | |a Proteins | |
| 650 | 4 | |a Immune response | |
| 650 | 4 | |a Vaccines | |
| 700 | 1 | |a BAI, XUE |4 oth | |
| 700 | 1 | |a WANG, LI BO |4 oth | |
| 700 | 1 | |a SHI, HAI NING |4 oth | |
| 700 | 1 | |a VAN DER GIESSEN, JOKE W. B |4 oth | |
| 700 | 1 | |a BOIREAU, PASCAL |4 oth | |
| 700 | 1 | |a LIU, MING YUAN |4 oth | |
| 700 | 1 | |a LIU, XIAO LEI |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Parasitology |d London [u.a.] : Cambridge Univ. Press, 1908 |g 144(2017), 6, Seite 1-8 |w (DE-627)129497770 |w (DE-600)207627-5 |w (DE-576)014896915 |x 0031-1820 |7 nnns |
| 773 | 1 | 8 | |g volume:144 |g year:2017 |g number:6 |g pages:1-8 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1017/S0031182016002493 |3 Volltext |
| 856 | 4 | 2 | |u https://search.proquest.com/docview/1898589786 |
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| 952 | |d 144 |j 2017 |e 6 |h 1-8 | ||