Acute West Nile Virus Meningoencephalitis Diagnosed Via Metagenomic Deep Sequencing of Cerebrospinal Fluid in a Renal Transplant Patient
Solid organ transplant patients are vulnerable to suffering neurologic complications from a wide array of viral infections and can be sentinels in the population who are first to get serious complications from emerging infections like the recent waves of arboviruses, including West Nile virus, Chikungunya virus, Zika virus, and Dengue virus. The diverse and rapidly changing landscape of possible causes of viral encephalitis poses great challenges for traditional candidate‐based infectious disease diagnostics that already fail to identify a causative pathogen in approximately 50% of encephalitis cases. We present the case of a 14‐year‐old girl on immunosuppression for a renal transplant who presented with acute meningoencephalitis. Traditional diagnostics failed to identify an etiology. RNA extracted from her cerebrospinal fluid was subjected to unbiased metagenomic deep sequencing, enhanced with the use of a Cas9‐based technique for host depletion. This analysis identified West Nile virus ( WNV ). Convalescent serum serologies subsequently confirmed WNV seroconversion. These results support a clear clinical role for metagenomic deep sequencing in the setting of suspected viral encephalitis, especially in the context of the high‐risk transplant patient population. Unbiased metagenomic deep sequencing identifies West Nile virus in the cerebrospinal fluid of a child who had a renal transplant and presented with acute meningoencephalitis..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
American journal of transplantation - 17(2017), 3, Seite 803-808 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wilson, M. R [VerfasserIn] |
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| Links: |
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| doi: |
10.1111/ajt.14058 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Acute West Nile Virus Meningoencephalitis Diagnosed Via Metagenomic Deep Sequencing of Cerebrospinal Fluid in a Renal Transplant Patient |
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| 520 | |a Solid organ transplant patients are vulnerable to suffering neurologic complications from a wide array of viral infections and can be sentinels in the population who are first to get serious complications from emerging infections like the recent waves of arboviruses, including West Nile virus, Chikungunya virus, Zika virus, and Dengue virus. The diverse and rapidly changing landscape of possible causes of viral encephalitis poses great challenges for traditional candidate‐based infectious disease diagnostics that already fail to identify a causative pathogen in approximately 50% of encephalitis cases. We present the case of a 14‐year‐old girl on immunosuppression for a renal transplant who presented with acute meningoencephalitis. Traditional diagnostics failed to identify an etiology. RNA extracted from her cerebrospinal fluid was subjected to unbiased metagenomic deep sequencing, enhanced with the use of a Cas9‐based technique for host depletion. This analysis identified West Nile virus ( WNV ). Convalescent serum serologies subsequently confirmed WNV seroconversion. These results support a clear clinical role for metagenomic deep sequencing in the setting of suspected viral encephalitis, especially in the context of the high‐risk transplant patient population. Unbiased metagenomic deep sequencing identifies West Nile virus in the cerebrospinal fluid of a child who had a renal transplant and presented with acute meningoencephalitis. | ||
| 540 | |a Nutzungsrecht: © 2016 The Authors. American Journal of Transplantation published by Wiley Periodicals, Inc. on behalf of American Society of Transplant Surgeons | ||
| 650 | 4 | |a genetics | |
| 650 | 4 | |a diagnostic techniques and imaging | |
| 650 | 4 | |a infectious disease | |
| 650 | 4 | |a viral: West Nile, infection and infectious agents | |
| 650 | 4 | |a basic (laboratory) research/science | |
| 650 | 4 | |a kidney (allograft) function/dysfunction | |
| 650 | 4 | |a clinical research/practice | |
| 650 | 4 | |a genomics | |
| 650 | 4 | |a viral: Epstein‐Barr Virus | |
| 650 | 4 | |a EBV | |
| 650 | 4 | |a infection and infectious agents | |
| 650 | 4 | |a kidney transplantation/nephrology | |
| 650 | 4 | |a Viral infections | |
| 650 | 4 | |a Health risk assessment | |
| 650 | 4 | |a West Nile virus | |
| 650 | 4 | |a Encephalitis | |
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| 700 | 1 | |a Crawford, E. D |4 oth | |
| 700 | 1 | |a Sample, H. A |4 oth | |
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| 700 | 1 | |a Baker, A. N |4 oth | |
| 700 | 1 | |a Khan, L. M |4 oth | |
| 700 | 1 | |a DeRisi, J. L |4 oth | |
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