An assessment of early colonisation of implant-abutment metal surfaces by single species and co-cultured bacterial periodontal pathogens

Objective Numerous studies have proposed that smooth metal surfaces reduce initial bacterial attachment in the establishment of an early biofilm formation. However, these studies have largely examined single bacterial species, which are not always relevant as pathogens identified as initiators of inflammatoryperi-implantitis. This study investigated the adherence of four periodontally-relevant bacterial species to implant and abutment surfaces in current clinical use. Methods Discs of polished cobalt chromium (CoCr-polished) and milled titanium (Ti-milled), representing two clinically relevant surfaces, were prepared and surfaces were characterised. Bacterial speciesPorphyromonas gingivalis,Fusobacterium nucleatum,Prevotella intermediaandAggregatibacter actinomycetemcomitanswere cultured to mid-log or stationary growth phase. Co-cultures ofP. gingivalis,F. nucleatumandP. gingivalis,F. nucleatum,Pr. intermediawere similarly prepared. Bacteria were inoculated onto discs for 2h, stained with a live/dead fluorescent stain and percentage bacterial coverage was calculated by confocal microscopy and image analysis. Results CoCr-polished discs had smooth surfaces with gentle valley structures, whilst Ti-milled discs had sharp edged peaks. Both discs demonstrated a partial wetting ability capable of initiating bacterial adhesion.P. gingivalis,F. nucleatumand co-cultures, at both mid-log and stationary concentrations, demonstrated equally high coverage of both the smooth CoCr-polished and the rougher Ti-milled metal surfaces.Pr. intermediaandA. actinomycetemcomitansdemonstrated lower surface coverage which was slightly higher for Ti-milled. Conclusion Variability was noted in the adherence potential for the respective periodontal pathogens examined. Particularly high adherence was noted forP. gingivalisandF. nucleatum, despite the manufacture of a smooth surface. Clinical significance Both surfaces studied may be used at implant-abutment junctions and both possess an ability to establish a bacterial biofilm containing a periodontally-relevant species. These surfaces are thus able to facilitate the apical migration of bacteria associated withperi-implantitis..

Medienart:	Artikel

Erscheinungsjahr:	2016
Erschienen:	2016

Enthalten in:	Zur Gesamtaufnahme - volume:53
Enthalten in:	Journal of dentistry - 53(2016), Seite 64-72

Sprache:	Englisch

Beteiligte Personen:	Jordan, Rachael P.C [VerfasserIn] Marsh, Lucy [Sonstige Person] Ayre, Wayne Nishio [Sonstige Person] Jones, Quentin [Sonstige Person] Parkes, Matthew [Sonstige Person] Austin, Bryan [Sonstige Person] Sloan, Alastair J [Sonstige Person] Waddington, Rachel J [Sonstige Person]

Links:	Volltext search.proquest.com

BKL:	44.96
Themen:	Bacteria Biofilms Carbon dioxide Pathogens Studies Transplants & implants

doi:	10.1016/j.jdent.2016.07.013

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	OLC1981776095