Inhibition of mitochondrial calcium uniporter protects neurocytes from ischemia/reperfusion injury via the inhibition of excessive mitophagy
Mitophagy plays an important role in mitochondrial quality control and cell survival during the process of ischemia/reperfusion (I/R) injury. Mitochondrial calcium uniporter (MCU) is the most important channel responsible for Ca(2+) influx into mitochondria and Ca(2+) signal plays a potential role in modulating mitophagy. However, the effect of MCU on mitophagy during the process of I/R injury remains unknown. This study constructed an in vitro I/R model by subjecting oxygen and glucose deprivation/reperfusion (OGD/RP) model to SH-SY5Y cells to mimic the cerebral I/R injury and aimed to explore the exact effect of MCU on I/R induced mitophagy. The results showed that OGD/RP induced autophagy and mitophagy in SH-SY5Y cells. Ru360, the inhibitor of MCU, improved mitochondrial morphology and fuctional stability as well as cell viability, significantly reduced OGD/RP induced mitophagy as evidenced by the decrease in Beclin-1 and the increase in Tom20 and P62 expression. Whereas spermine, the agonist of MCU, had no significant impact on the expression of those mitophagy related proteins compared with OGD/RP group. This study indicates that inhibition of MCU can inhibit excessive mitophagy and protect the neurocytes from I/R injury..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:628 |
|---|---|
| Enthalten in: |
Neuroscience letters - 628(2016), Seite 24-29 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yu, Shoushui [VerfasserIn] |
|---|
| Links: |
|---|
| BKL: |
|---|
| doi: |
10.1016/j.neulet.2016.06.012 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC1980308845 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1980308845 | ||
| 003 | DE-627 | ||
| 005 | 20230516135116.0 | ||
| 007 | tu | ||
| 008 | 160816s2016 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.neulet.2016.06.012 |2 doi | |
| 028 | 5 | 2 | |a PQ20160815 |
| 035 | |a (DE-627)OLC1980308845 | ||
| 035 | |a (DE-599)GBVOLC1980308845 | ||
| 035 | |a (PRQ)c926-cb27abed57014afb9dc4c657e14eba0187718e726e1b6d39961a8ab33c4c0a2d0 | ||
| 035 | |a (KEY)0111962120160000628000000024inhibitionofmitochondrialcalciumuniporterprotectsn | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 590 |a 610 |q DNB |
| 084 | |a BIODIV |2 fid | ||
| 084 | |a 44.90 |2 bkl | ||
| 084 | |a 42.63 |2 bkl | ||
| 100 | 1 | |a Yu, Shoushui |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibition of mitochondrial calcium uniporter protects neurocytes from ischemia/reperfusion injury via the inhibition of excessive mitophagy |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a Mitophagy plays an important role in mitochondrial quality control and cell survival during the process of ischemia/reperfusion (I/R) injury. Mitochondrial calcium uniporter (MCU) is the most important channel responsible for Ca(2+) influx into mitochondria and Ca(2+) signal plays a potential role in modulating mitophagy. However, the effect of MCU on mitophagy during the process of I/R injury remains unknown. This study constructed an in vitro I/R model by subjecting oxygen and glucose deprivation/reperfusion (OGD/RP) model to SH-SY5Y cells to mimic the cerebral I/R injury and aimed to explore the exact effect of MCU on I/R induced mitophagy. The results showed that OGD/RP induced autophagy and mitophagy in SH-SY5Y cells. Ru360, the inhibitor of MCU, improved mitochondrial morphology and fuctional stability as well as cell viability, significantly reduced OGD/RP induced mitophagy as evidenced by the decrease in Beclin-1 and the increase in Tom20 and P62 expression. Whereas spermine, the agonist of MCU, had no significant impact on the expression of those mitophagy related proteins compared with OGD/RP group. This study indicates that inhibition of MCU can inhibit excessive mitophagy and protect the neurocytes from I/R injury. | ||
| 540 | |a Nutzungsrecht: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. | ||
| 700 | 1 | |a Zheng, Shengfa |4 oth | |
| 700 | 1 | |a Leng, Jing |4 oth | |
| 700 | 1 | |a Wang, Shilei |4 oth | |
| 700 | 1 | |a Zhao, Tao |4 oth | |
| 700 | 1 | |a Liu, Jia |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Neuroscience letters |d Amsterdam : Elsevier, 1975 |g 628(2016), Seite 24-29 |w (DE-627)129441031 |w (DE-600)194929-9 |w (DE-576)014810395 |x 0304-3940 |7 nnns |
| 773 | 1 | 8 | |g volume:628 |g year:2016 |g pages:24-29 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.neulet.2016.06.012 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/27288019 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-PSY | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4219 | ||
| 912 | |a GBV_ILN_4305 | ||
| 936 | b | k | |a 44.90 |q AVZ |
| 936 | b | k | |a 42.63 |q AVZ |
| 951 | |a AR | ||
| 952 | |d 628 |j 2016 |h 24-29 | ||