MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation
MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors. Previous studies of MAGE-A1 in melanoma mainly focused on methylation changes or its role in immunotherapy, however, its biological functions in melanoma have remained unknown. In order to determine the role of MAGE-A1 in melanoma growth and metastasis, we manipulated melanoma cell lines with overexpression and knockdown of MAGE-A1. Integration of cell proliferation assays, transwell migration and invasion assays, and RNA-Seq analysis revealed that up-regulation of MAGE-A1 dramatically promoted proliferation, migration, and invasion of human melanoma cell lines in vitro, while down-regulation of MAGE-A1 inhibited those characteristics associated with tumor cells. Furthermore, transcriptome sequencing revealed that MAGE-A1 exerts its tumor promoting activity by activating p-C-JUN directly or through ERK-MAPK signaling pathways. Based on our findings, we propose that MAGE-A1 may be a potential therapeutic target for melanoma patients..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:473 |
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| Enthalten in: |
Biochemical and biophysical research communications - 473(2016), 4, Seite 959-965 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Dong [VerfasserIn] |
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| doi: |
10.1016/j.bbrc.2016.03.161 |
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| 520 | |a MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors. Previous studies of MAGE-A1 in melanoma mainly focused on methylation changes or its role in immunotherapy, however, its biological functions in melanoma have remained unknown. In order to determine the role of MAGE-A1 in melanoma growth and metastasis, we manipulated melanoma cell lines with overexpression and knockdown of MAGE-A1. Integration of cell proliferation assays, transwell migration and invasion assays, and RNA-Seq analysis revealed that up-regulation of MAGE-A1 dramatically promoted proliferation, migration, and invasion of human melanoma cell lines in vitro, while down-regulation of MAGE-A1 inhibited those characteristics associated with tumor cells. Furthermore, transcriptome sequencing revealed that MAGE-A1 exerts its tumor promoting activity by activating p-C-JUN directly or through ERK-MAPK signaling pathways. Based on our findings, we propose that MAGE-A1 may be a potential therapeutic target for melanoma patients. | ||
| 540 | |a Nutzungsrecht: Copyright © 2016. Published by Elsevier Inc. | ||
| 700 | 1 | |a Wang, Junyun |4 oth | |
| 700 | 1 | |a Ding, Nan |4 oth | |
| 700 | 1 | |a Li, Yongjun |4 oth | |
| 700 | 1 | |a Yang, Yaran |4 oth | |
| 700 | 1 | |a Fang, Xiangdong |4 oth | |
| 700 | 1 | |a Zhao, Hua |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d San Diego, Calif. : Elsevier, 1959 |g 473(2016), 4, Seite 959-965 |w (DE-627)129488798 |w (DE-600)205723-2 |w (DE-576)014881640 |x 0006-291X |7 nnns |
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| 856 | 4 | 2 | |u http://www.sciencedirect.com/science/article/pii/S0006291X16304831 |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/27045082 |
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