TRPV4 is endogenously expressed in vertebrate spermatozoa and regulates intracellular calcium in human sperm
Transient Receptor Potential Vanilloid sub-type 4 (TRPV4) is a non-selective cationic channel involved in regulation of temperature, osmolality and different ligand-dependent Ca(2+)-influx. Recently, we have demonstrated that TRPV4 is conserved in all vertebrates. Now we demonstrate that TRPV4 is endogenously expressed in all vertebrate sperm cells ranging from fish to mammals. In human sperm, TRPV4 is present as N-glycosylated protein and its activation induces Ca(2+)-influx. Its expression and localization differs in swim-up and swim-down cells suggesting that TRPV4 is an important determining factor for sperm motility. We demonstrate that pharmacological activation or inhibition of TRPV4 regulates Ca(2+)-wave propagation from head to tail. Such findings may have wide application in male fertility-infertility, contraception and conservation of endangered species as well..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:473 |
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| Enthalten in: |
Biochemical and biophysical research communications - 473(2016), 4, Seite 781-788 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kumar, Ashutosh [VerfasserIn] |
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| doi: |
10.1016/j.bbrc.2016.03.071 |
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| 245 | 1 | 0 | |a TRPV4 is endogenously expressed in vertebrate spermatozoa and regulates intracellular calcium in human sperm |
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| 520 | |a Transient Receptor Potential Vanilloid sub-type 4 (TRPV4) is a non-selective cationic channel involved in regulation of temperature, osmolality and different ligand-dependent Ca(2+)-influx. Recently, we have demonstrated that TRPV4 is conserved in all vertebrates. Now we demonstrate that TRPV4 is endogenously expressed in all vertebrate sperm cells ranging from fish to mammals. In human sperm, TRPV4 is present as N-glycosylated protein and its activation induces Ca(2+)-influx. Its expression and localization differs in swim-up and swim-down cells suggesting that TRPV4 is an important determining factor for sperm motility. We demonstrate that pharmacological activation or inhibition of TRPV4 regulates Ca(2+)-wave propagation from head to tail. Such findings may have wide application in male fertility-infertility, contraception and conservation of endangered species as well. | ||
| 540 | |a Nutzungsrecht: © Elsevier Inc. | ||
| 540 | |a Copyright © 2016 Elsevier Inc. All rights reserved. | ||
| 700 | 1 | |a Majhi, Rakesh Kumar |4 oth | |
| 700 | 1 | |a Swain, Nirlipta |4 oth | |
| 700 | 1 | |a Giri, S.C |4 oth | |
| 700 | 1 | |a Kar, Sujata |4 oth | |
| 700 | 1 | |a Samanta, Luna |4 oth | |
| 700 | 1 | |a Goswami, Chandan |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d San Diego, Calif. : Elsevier, 1959 |g 473(2016), 4, Seite 781-788 |w (DE-627)129488798 |w (DE-600)205723-2 |w (DE-576)014881640 |x 0006-291X |7 nnns |
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| 856 | 4 | 2 | |u http://www.sciencedirect.com/science/article/pii/S0006291X16303825 |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/27003252 |
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