The molybdenum cofactor biosynthesis complex interacts with actin filaments via molybdenum insertase Cnx1 as anchor protein in Arabidopsis thaliana
The pterin based molybdenum cofactor (Moco) plays an essential role in almost all organisms. Its biosynthesis is catalysed by six enzymes in a conserved four step reaction pathway. The last three steps are located in the cytoplasm, where a multimeric protein complex is formed to protect the intermediates from degradation. Bimolecular fluorescence complementation was used to test for cytoskeleton association of the Moco biosynthesis enzymes with actin filaments and microtubules using known cytoskeleton associated proteins, thus permitting non-invasive in vivo studies. Coding sequences of binding proteins were cloned via the GATEWAY system. No Moco biosynthesis enzyme showed any interaction with microtubules. However, alone the two domain protein Cnx1 exhibited interaction with actin filaments mediated by both domains with the Cnx1G domain displaying a stronger interaction. Cnx6 showed actin association only if unlabelled Cnx1 was co-expressed in comparable amounts. So Cnx1 is likely to be the anchor protein for the whole biosynthesis complex on actin filaments. A stabilization of the whole Moco biosynthesis complex on the cytoskeleton might be crucial. In addition a micro-compartmentation might either allow a localisation near the mitochondrial ATM3 exporter providing the first Moco intermediate or near one of the three molybdate transporters enabling efficient molybdate incorporation..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:244 |
|---|---|
| Enthalten in: |
Plant science - 244(2016), Seite 8-18 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kaufholdt, David [VerfasserIn] |
|---|
| Links: |
|---|
| BKL: |
|---|
| RVK: |
|---|
| doi: |
10.1016/j.plantsci.2015.12.011 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC1972827391 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1972827391 | ||
| 003 | DE-627 | ||
| 005 | 20220219174551.0 | ||
| 007 | tu | ||
| 008 | 160427s2016 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.plantsci.2015.12.011 |2 doi | |
| 028 | 5 | 2 | |a PQ20160430 |
| 035 | |a (DE-627)OLC1972827391 | ||
| 035 | |a (DE-599)GBVOLC1972827391 | ||
| 035 | |a (PRQ)c949-6f6724292e8fdffc6a124017446539761a8f333586aff2a1b57f2c39b5c2dbab0 | ||
| 035 | |a (KEY)0065055220160000244000000008molybdenumcofactorbiosynthesiscomplexinteractswith | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 580 |a 570 |q DNB |
| 084 | |a BIODIV |2 fid | ||
| 084 | |a WA 15000 |q AVZ |2 rvk | ||
| 084 | |a 48.50 |2 bkl | ||
| 084 | |a 42.38 |2 bkl | ||
| 100 | 1 | |a Kaufholdt, David |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The molybdenum cofactor biosynthesis complex interacts with actin filaments via molybdenum insertase Cnx1 as anchor protein in Arabidopsis thaliana |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a The pterin based molybdenum cofactor (Moco) plays an essential role in almost all organisms. Its biosynthesis is catalysed by six enzymes in a conserved four step reaction pathway. The last three steps are located in the cytoplasm, where a multimeric protein complex is formed to protect the intermediates from degradation. Bimolecular fluorescence complementation was used to test for cytoskeleton association of the Moco biosynthesis enzymes with actin filaments and microtubules using known cytoskeleton associated proteins, thus permitting non-invasive in vivo studies. Coding sequences of binding proteins were cloned via the GATEWAY system. No Moco biosynthesis enzyme showed any interaction with microtubules. However, alone the two domain protein Cnx1 exhibited interaction with actin filaments mediated by both domains with the Cnx1G domain displaying a stronger interaction. Cnx6 showed actin association only if unlabelled Cnx1 was co-expressed in comparable amounts. So Cnx1 is likely to be the anchor protein for the whole biosynthesis complex on actin filaments. A stabilization of the whole Moco biosynthesis complex on the cytoskeleton might be crucial. In addition a micro-compartmentation might either allow a localisation near the mitochondrial ATM3 exporter providing the first Moco intermediate or near one of the three molybdate transporters enabling efficient molybdate incorporation. | ||
| 540 | |a Nutzungsrecht: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. | ||
| 700 | 1 | |a Baillie, Christin-Kirsty |4 oth | |
| 700 | 1 | |a Bikker, Rolf |4 oth | |
| 700 | 1 | |a Burkart, Valentin |4 oth | |
| 700 | 1 | |a Dudek, Christian-Alexander |4 oth | |
| 700 | 1 | |a Pein, Linn von |4 oth | |
| 700 | 1 | |a Rothkegel, Martin |4 oth | |
| 700 | 1 | |a Mendel, Ralf R |4 oth | |
| 700 | 1 | |a Hänsch, Robert |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Plant science |d Amsterdam [u.a.] : Elsevier, 1985 |g 244(2016), Seite 8-18 |w (DE-627)13046838X |w (DE-600)742010-9 |w (DE-576)016061578 |x 0168-9452 |7 nnns |
| 773 | 1 | 8 | |g volume:244 |g year:2016 |g pages:8-18 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.plantsci.2015.12.011 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/26810449 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-FOR | ||
| 912 | |a GBV_ILN_21 | ||
| 912 | |a GBV_ILN_70 | ||
| 936 | r | v | |a WA 15000 |
| 936 | b | k | |a 48.50 |q AVZ |
| 936 | b | k | |a 42.38 |q AVZ |
| 951 | |a AR | ||
| 952 | |d 244 |j 2016 |h 8-18 | ||