Anti-inflammatory and antioxidant properties of unripe papaya extract in an excision wound model
Abstract Context: Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties. Objectives: We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition. Materials and methods: Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se2+ added PE (PES), or 100 µM Zn2+ added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding. Results: PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se2+; 8.90 ± 0.23 d) significantly (p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study. Discussion and conclusion: Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes..
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Artikel |
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Erscheinungsjahr: |
2015 |
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Erschienen: |
2015 |
Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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Enthalten in: |
Pharmaceutical biology - 53(2015), 5, Seite 662-671 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Nafiu, Abdulrazaq Bidemi [VerfasserIn] |
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Links: |
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doi: |
10.3109/13880209.2014.936470 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
OLC1966537026 |
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520 | |a Abstract Context: Carica papaya L. (Caricaceae) fruit was shown to exhibit wound healing properties. Objectives: We investigated anti-inflammatory and antioxidant potential of papaya fruit phosphate-buffered saline extract (PE) during wound healing and enhancement of the potentials due to trace ions addition. Materials and methods: Rat excision wounds were topically treated twice/day with 20 µL of PE (5 mg extract/mL), 0.5 µg Se2+ added PE (PES), or 100 µM Zn2+ added PE (PEZ). Control groups were treated with deionized water (negative) and deproteinized calf blood extract ointment (Solcoseryl®, positive). Lipid peroxidation (LPX), antioxidant, proinflammatory, and arginine metabolic enzymes were estimated in the wound excised on days 4 and 10 post wounding. Results: PE (5 mg/mL; 9.80 ± 0.33 d) and PES (PE + 0.5 µg Se2+; 8.90 ± 0.23 d) significantly (p < 0.05) reduced the average time for complete wound closure compared with the negative (13.00 ± 0.37 d) and positive (9.80 ± 0.33 d) controls, respectively. Biochemical evaluations of LPX product (malondialdehyde), antioxidant (catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx)), and pro-inflammatory (cyclooxygenase-2 and myeloperoxidase (MPO)) enzyme activities and metabolites (nitrite and urea), on days 4 and 10 post wounding, confirmed the anti-inflammatory and antioxidant properties of PE and PES in this study. Discussion and conclusion: Treatment of excision wounds with papaya extract, especially with the addition of selenium for 10 d, reduced inflammation associated oxidative damage apparently via cyclooxygenase specific inhibition, arginine metabolism, and up-regulation of antioxidant enzymes. | ||
540 | |a Nutzungsrecht: © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted | ||
650 | 4 | |a Antioxidants - isolation & purification | |
650 | 4 | |a Plant Extracts - isolation & purification | |
650 | 4 | |a Lipid Peroxidation - physiology | |
650 | 4 | |a Plant Extracts - pharmacology | |
650 | 4 | |a Wound Healing - physiology | |
650 | 4 | |a Lipid Peroxidation - drug effects | |
650 | 4 | |a Antioxidants - pharmacology | |
650 | 4 | |a Wound Healing - drug effects | |
650 | 4 | |a Anti-Inflammatory Agents - pharmacology | |
650 | 4 | |a Anti-Inflammatory Agents - isolation & purification | |
700 | 1 | |a Rahman, Mohammad Tariqur |4 oth | |
773 | 0 | 8 | |i Enthalten in |t Pharmaceutical biology |d New York, NY : Informa Healthcare, 1998 |g 53(2015), 5, Seite 662-671 |w (DE-627)247468169 |w (DE-600)1440131-9 |w (DE-576)435515888 |x 1388-0209 |7 nnns |
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