Escherichia coli‐induced immune paralysis is not exacerbated during chronic filarial infection
Sepsis initially starts with a systemic inflammatory response ( SIRS phase) and is followed by a compensatory anti‐inflammatory response syndrome ( CARS ) that causes impaired adaptive T‐cell immunity, immune paralysis and an increased susceptibility to secondary infections. In contrast, parasitic filariae release thousands of microfilariae into the peripheral blood without triggering inflammation, as they induce regulatory, anti‐inflammatory host responses. Hence, we investigated the impact of chronic filarial infection on adaptive T‐cell responses during the SIRS and CARS phases of a systemic bacterial infection and analysed the development of T‐cell paralysis following a subsequent adenovirus challenge in BALB /c mice. Chronic filarial infection impaired adenovirus‐specific CD 8 + T‐cell cytotoxicity and interferon‐ γ responses in the absence of a bacterial challenge and led to higher numbers of splenic CTLA ‐4 + CD 4 + T cells, whereas splenic T‐cell expression of CD 69 and CD 62 ligand, serum cytokine levels and regulatory T‐cell frequencies were comparable to naive controls. Irrespective of filarial infection, the SIRS phase dominated 6–24 hr after intravenous Escherichia coli challenge with increased T‐cell activation and pro‐inflammatory cytokine production, whereas the CARS phase occurred 6 days post E. coli challenge and correlated with high levels of transforming growth factor‐ β and increased CD 62 ligand T‐cell expression. Escherichia coli ‐induced impairment of adenovirus‐specific CD 8 + T‐cell cytotoxicity and interferon‐ γ production was not additionally impaired by chronic filarial infection. This suggests that filarial immunoregulation does not exacerbate E. coli ‐induced T‐cell paralysis..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:145 |
|---|---|
| Enthalten in: |
Immunology - 145(2015), 1, Seite 150-160 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Buerfent, Benedikt C [VerfasserIn] |
|---|
| Links: |
Volltext |
|---|
| RVK: |
|---|
| doi: |
10.1111/imm.12435 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC1964045754 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1964045754 | ||
| 003 | DE-627 | ||
| 005 | 20230516124132.0 | ||
| 007 | tu | ||
| 008 | 160206s2015 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1111/imm.12435 |2 doi | |
| 028 | 5 | 2 | |a PQ20160617 |
| 035 | |a (DE-627)OLC1964045754 | ||
| 035 | |a (DE-599)GBVOLC1964045754 | ||
| 035 | |a (PRQ)c2085-e20d1d047c7882694648ee9641005b7f229d916f560f5d6bb00dd594ca87308e0 | ||
| 035 | |a (KEY)0025421520150000145000100150escherichiacoliinducedimmuneparalysisisnotexacerba | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 610 |q DNB |
| 084 | |a XA 10000 |q AVZ |2 rvk | ||
| 084 | |a 44.45 |2 bkl | ||
| 100 | 1 | |a Buerfent, Benedikt C |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Escherichia coli‐induced immune paralysis is not exacerbated during chronic filarial infection |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a Sepsis initially starts with a systemic inflammatory response ( SIRS phase) and is followed by a compensatory anti‐inflammatory response syndrome ( CARS ) that causes impaired adaptive T‐cell immunity, immune paralysis and an increased susceptibility to secondary infections. In contrast, parasitic filariae release thousands of microfilariae into the peripheral blood without triggering inflammation, as they induce regulatory, anti‐inflammatory host responses. Hence, we investigated the impact of chronic filarial infection on adaptive T‐cell responses during the SIRS and CARS phases of a systemic bacterial infection and analysed the development of T‐cell paralysis following a subsequent adenovirus challenge in BALB /c mice. Chronic filarial infection impaired adenovirus‐specific CD 8 + T‐cell cytotoxicity and interferon‐ γ responses in the absence of a bacterial challenge and led to higher numbers of splenic CTLA ‐4 + CD 4 + T cells, whereas splenic T‐cell expression of CD 69 and CD 62 ligand, serum cytokine levels and regulatory T‐cell frequencies were comparable to naive controls. Irrespective of filarial infection, the SIRS phase dominated 6–24 hr after intravenous Escherichia coli challenge with increased T‐cell activation and pro‐inflammatory cytokine production, whereas the CARS phase occurred 6 days post E. coli challenge and correlated with high levels of transforming growth factor‐ β and increased CD 62 ligand T‐cell expression. Escherichia coli ‐induced impairment of adenovirus‐specific CD 8 + T‐cell cytotoxicity and interferon‐ γ production was not additionally impaired by chronic filarial infection. This suggests that filarial immunoregulation does not exacerbate E. coli ‐induced T‐cell paralysis. | ||
| 540 | |a Nutzungsrecht: © 2014 John Wiley & Sons Ltd | ||
| 540 | |a © 2014 John Wiley & Sons Ltd. | ||
| 650 | 4 | |a sepsis | |
| 650 | 4 | |a T‐cell cytotoxicity | |
| 650 | 4 | |a helminth | |
| 650 | 4 | |a immune paralysis | |
| 650 | 4 | |a compensatory anti‐inflammatory response syndrome | |
| 650 | 4 | |a Systemic Inflammatory Response Syndrome - immunology | |
| 650 | 4 | |a Interferon-gamma - genetics | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Systemic Inflammatory Response Syndrome - genetics | |
| 650 | 4 | |a Transforming Growth Factor beta - immunology | |
| 650 | 4 | |a Filarioidea - immunology | |
| 650 | 4 | |a Escherichia coli - immunology | |
| 650 | 4 | |a Filariasis - pathology | |
| 650 | 4 | |a Filariasis - immunology | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes - pathology | |
| 650 | 4 | |a Escherichia coli Infections - genetics | |
| 650 | 4 | |a Interferon-gamma - immunology | |
| 650 | 4 | |a Filariasis - genetics | |
| 650 | 4 | |a Escherichia coli Infections - pathology | |
| 650 | 4 | |a Systemic Inflammatory Response Syndrome - pathology | |
| 650 | 4 | |a CD4-Positive T-Lymphocytes - pathology | |
| 650 | 4 | |a CD4-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Transforming Growth Factor beta - genetics | |
| 650 | 4 | |a Escherichia coli Infections - immunology | |
| 700 | 1 | |a Gondorf, Fabian |4 oth | |
| 700 | 1 | |a Wohlleber, Dirk |4 oth | |
| 700 | 1 | |a Schumak, Beatrix |4 oth | |
| 700 | 1 | |a Hoerauf, Achim |4 oth | |
| 700 | 1 | |a Hübner, Marc P |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Immunology |d Oxford [u.a.] : Wiley-Blackwell, 1958 |g 145(2015), 1, Seite 150-160 |w (DE-627)129269271 |w (DE-600)80124-0 |w (DE-576)01445971X |x 0019-2805 |7 nnns |
| 773 | 1 | 8 | |g volume:145 |g year:2015 |g number:1 |g pages:150-160 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1111/imm.12435 |3 Volltext |
| 856 | 4 | 2 | |u http://onlinelibrary.wiley.com/doi/10.1111/imm.12435/abstract |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25521437 |
| 856 | 4 | 2 | |u http://search.proquest.com/docview/1672912204 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4219 | ||
| 936 | r | v | |a XA 10000 |
| 936 | b | k | |a 44.45 |q AVZ |
| 951 | |a AR | ||
| 952 | |d 145 |j 2015 |e 1 |h 150-160 | ||