Functional characterization of myeloid‐derived suppressor cell subpopulations during the development of experimental arthritis
Recent evidence indicates the existence of subpopulations of myeloid‐derived suppressor cells (MDSCs) with distinct phenotypes and functions. Here, we characterized the role of MDSC subpopulations in the pathogenesis of autoimmune arthritis in a collagen‐induced arthritis (CIA) mouse model. The splenic CD11b + Gr‐1 + MDSC population expanded in CIA mice, and these cells could be subdivided into polymorphonuclear (PMN) and mononuclear (MO) MDSC subpopulations based on Ly6C and Ly6G expression. During CIA, the proportion of splenic MO‐MDSCs was increased in association with the severity of joint inflammation, while PMN‐MDSCs were decreased. MO‐MDSCs expressed higher levels of surface CD40 and CD86 protein, but lower levels of Il10, Tgfb1, Ccr5, and Cxcr2 mRNA. PMN‐MDSCs exhibited a more potent capacity to suppress polyclonal T‐cell proliferation in vitro, compared with MO‐MDSCs. Moreover, the adoptive transfer of PMN‐MDSCs, but not MO‐MDSCs, decreased joint inflammation, accompanied by reduced levels of serum cytokine secretion and the frequencies of Th1 and Th17 cells in draining lymph nodes. These results suggest that there could be a shift from potently suppressive PMN‐MDSCs to poorly suppressive MO‐MDSCs during the development of experimental arthritis, which might reflect the failure of expanded MDSCs to suppress autoimmune arthritis..
Medienart: |
Artikel |
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Erscheinungsjahr: |
2015 |
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Erschienen: |
2015 |
Enthalten in: |
Zur Gesamtaufnahme - volume:45 |
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Enthalten in: |
European journal of immunology - 45(2015), 2, Seite 464-473 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Wang, Wenhong [VerfasserIn] |
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Links: |
Volltext |
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doi: |
10.1002/eji.201444799 |
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funding: |
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PPN (Katalog-ID): |
OLC196296874X |
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520 | |a Recent evidence indicates the existence of subpopulations of myeloid‐derived suppressor cells (MDSCs) with distinct phenotypes and functions. Here, we characterized the role of MDSC subpopulations in the pathogenesis of autoimmune arthritis in a collagen‐induced arthritis (CIA) mouse model. The splenic CD11b + Gr‐1 + MDSC population expanded in CIA mice, and these cells could be subdivided into polymorphonuclear (PMN) and mononuclear (MO) MDSC subpopulations based on Ly6C and Ly6G expression. During CIA, the proportion of splenic MO‐MDSCs was increased in association with the severity of joint inflammation, while PMN‐MDSCs were decreased. MO‐MDSCs expressed higher levels of surface CD40 and CD86 protein, but lower levels of Il10, Tgfb1, Ccr5, and Cxcr2 mRNA. PMN‐MDSCs exhibited a more potent capacity to suppress polyclonal T‐cell proliferation in vitro, compared with MO‐MDSCs. Moreover, the adoptive transfer of PMN‐MDSCs, but not MO‐MDSCs, decreased joint inflammation, accompanied by reduced levels of serum cytokine secretion and the frequencies of Th1 and Th17 cells in draining lymph nodes. These results suggest that there could be a shift from potently suppressive PMN‐MDSCs to poorly suppressive MO‐MDSCs during the development of experimental arthritis, which might reflect the failure of expanded MDSCs to suppress autoimmune arthritis. | ||
540 | |a Nutzungsrecht: © 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim | ||
540 | |a © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | ||
650 | 4 | |a Rheumatoid arthritis | |
650 | 4 | |a Myeloid‐derived suppressor cells | |
650 | 4 | |a Collagen‐induced arthritis | |
650 | 4 | |a T cells | |
650 | 4 | |a Lymph Nodes - immunology | |
650 | 4 | |a Antigens, Ly - genetics | |
650 | 4 | |a Th17 Cells - metabolism | |
650 | 4 | |a Arthritis, Experimental - pathology | |
650 | 4 | |a Myeloid Cells - immunology | |
650 | 4 | |a CD8-Positive T-Lymphocytes - metabolism | |
650 | 4 | |a Th1 Cells - pathology | |
650 | 4 | |a Antigens, Ly - immunology | |
650 | 4 | |a Cell Lineage - immunology | |
650 | 4 | |a Antigens, CD11b - genetics | |
650 | 4 | |a Antigens, CD11b - immunology | |
650 | 4 | |a Joints - pathology | |
650 | 4 | |a Lymph Nodes - pathology | |
650 | 4 | |a Antigens, CD86 - genetics | |
650 | 4 | |a CD8-Positive T-Lymphocytes - immunology | |
650 | 4 | |a Receptors, Chemokine - immunology | |
650 | 4 | |a Th1 Cells - immunology | |
650 | 4 | |a Arthritis, Experimental - immunology | |
650 | 4 | |a Th17 Cells - immunology | |
650 | 4 | |a CD8-Positive T-Lymphocytes - pathology | |
650 | 4 | |a Joints - immunology | |
650 | 4 | |a Myeloid Cells - pathology | |
650 | 4 | |a Spleen - pathology | |
650 | 4 | |a Antigens, CD40 - genetics | |
650 | 4 | |a Receptors, Chemokine - genetics | |
650 | 4 | |a Spleen - immunology | |
650 | 4 | |a Arthritis, Experimental - genetics | |
650 | 4 | |a Th1 Cells - metabolism | |
650 | 4 | |a Antigens, CD40 - immunology | |
650 | 4 | |a Antigens, CD86 - immunology | |
650 | 4 | |a Th17 Cells - pathology | |
650 | 4 | |a Myeloid Cells - transplantation | |
650 | 4 | |a Leucocytes | |
650 | 4 | |a Medical research | |
650 | 4 | |a Arthritis | |
650 | 4 | |a Rodents | |
700 | 1 | |a Jiao, Zhijun |4 oth | |
700 | 1 | |a Duan, Tanghai |4 oth | |
700 | 1 | |a Liu, Meihong |4 oth | |
700 | 1 | |a Zhu, Bo |4 oth | |
700 | 1 | |a Zhang, Yingying |4 oth | |
700 | 1 | |a Xu, Qiugui |4 oth | |
700 | 1 | |a Wang, Rui |4 oth | |
700 | 1 | |a Xiong, Yuyun |4 oth | |
700 | 1 | |a Xu, Huaxi |4 oth | |
700 | 1 | |a Lu, Liwei |4 oth | |
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856 | 4 | 1 | |u http://dx.doi.org/10.1002/eji.201444799 |3 Volltext |
856 | 4 | 2 | |u http://onlinelibrary.wiley.com/doi/10.1002/eji.201444799/abstract |
856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25352399 |
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