Molecular epidemiology of carbapenem non-susceptible Acinetobacter nosocomialis in a medical center in Taiwan
The mechanism by which carbapenem non-susceptible Acinetobacter nosocomialis (CNSAN) is disseminated is rarely described in the literature. In this study, we delineated the molecular epidemiology of CNSAN isolated from patients in a medical center in Taiwan. Fifty-four non-duplicate bloodstream isolates of CNSAN were collected at the Taipei Veterans General Hospital between 2001 and 2007. Pulsed-field gel electrophoresis (PFGE) was performed to determine their clonal relationship. Carbapenem-resistance genes and associated genetic structures were detected by polymerase chain reaction (PCR) mapping. Southern hybridization was performed to determine the plasmid location of carbapenem-resistance genes. Transmissibility of these genes to Acinetobacterbaumannii was demonstrated by conjugation tests. The overall carbapenem non-susceptibility rate among A. nosocomialis isolates during the study period was 21.6% (54/250). PFGE revealed three major pulsotypes: H (n=23), I (n=10), and K (n=8). The most common carbapenem-resistance gene was blaOXA-58 (43/54, 79.6%), containing an upstream insertion sequence IS1006 and a truncated ISAba3 (IS1006-ΔISAba3-like-blaOXA-58). All isolates belonging to the pulsotypes H, I, and K carried plasmid located IS1006-ΔISAba3-like-blaOXA-58. A common plasmid carrying ISAba1-blaOXA-82 was found in six isolates, which belonged to five pulsotypes. A type 1 integron that carried blaIMP-1 was detected in different plasmids of seven isolates, which belonged to five pulsotypes. Plasmids carrying these carbapenem-resistant determinants were transmissible from A. nosocomialis to A. baumannii via conjugation. In this medical center, CNSAN mainly emerged through clonal dissemination; propagation of plasmids and integrons carrying carbapenem-resistant determinants played a minor role. This study showed that plasmids carrying carbapenem-resistant determinants are transmissible from A. nosocomialis to A. baumannii..
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Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
Infection, genetics and evolution - 31(2015), Seite 305-311 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Ya-Sung [VerfasserIn] |
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| Links: |
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| doi: |
10.1016/j.meegid.2015.02.017 |
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| 520 | |a The mechanism by which carbapenem non-susceptible Acinetobacter nosocomialis (CNSAN) is disseminated is rarely described in the literature. In this study, we delineated the molecular epidemiology of CNSAN isolated from patients in a medical center in Taiwan. Fifty-four non-duplicate bloodstream isolates of CNSAN were collected at the Taipei Veterans General Hospital between 2001 and 2007. Pulsed-field gel electrophoresis (PFGE) was performed to determine their clonal relationship. Carbapenem-resistance genes and associated genetic structures were detected by polymerase chain reaction (PCR) mapping. Southern hybridization was performed to determine the plasmid location of carbapenem-resistance genes. Transmissibility of these genes to Acinetobacterbaumannii was demonstrated by conjugation tests. The overall carbapenem non-susceptibility rate among A. nosocomialis isolates during the study period was 21.6% (54/250). PFGE revealed three major pulsotypes: H (n=23), I (n=10), and K (n=8). The most common carbapenem-resistance gene was blaOXA-58 (43/54, 79.6%), containing an upstream insertion sequence IS1006 and a truncated ISAba3 (IS1006-ΔISAba3-like-blaOXA-58). All isolates belonging to the pulsotypes H, I, and K carried plasmid located IS1006-ΔISAba3-like-blaOXA-58. A common plasmid carrying ISAba1-blaOXA-82 was found in six isolates, which belonged to five pulsotypes. A type 1 integron that carried blaIMP-1 was detected in different plasmids of seven isolates, which belonged to five pulsotypes. Plasmids carrying these carbapenem-resistant determinants were transmissible from A. nosocomialis to A. baumannii via conjugation. In this medical center, CNSAN mainly emerged through clonal dissemination; propagation of plasmids and integrons carrying carbapenem-resistant determinants played a minor role. This study showed that plasmids carrying carbapenem-resistant determinants are transmissible from A. nosocomialis to A. baumannii. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 Elsevier B.V. All rights reserved. | ||
| 650 | 4 | |a Acinetobacter - isolation & purification | |
| 650 | 4 | |a Acinetobacter Infections - epidemiology | |
| 650 | 4 | |a Acinetobacter Infections - microbiology | |
| 650 | 4 | |a Acinetobacter - drug effects | |
| 650 | 4 | |a Acinetobacter - genetics | |
| 650 | 4 | |a Acinetobacter - classification | |
| 650 | 4 | |a Carbapenems - pharmacology | |
| 700 | 1 | |a Lee, Yi-Tzu |4 oth | |
| 700 | 1 | |a Wang, Yung-Chih |4 oth | |
| 700 | 1 | |a Chiu, Chun-Hsiang |4 oth | |
| 700 | 1 | |a Kuo, Shu-Chen |4 oth | |
| 700 | 1 | |a Sun, Jun-Ren |4 oth | |
| 700 | 1 | |a Yin, Ti |4 oth | |
| 700 | 1 | |a Chen, Te-Li |4 oth | |
| 700 | 1 | |a Lin, Jung-Chung |4 oth | |
| 700 | 1 | |a Fung, Chang-Phone |4 oth | |
| 700 | 1 | |a Chang, Feng-Yee |4 oth | |
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| 773 | 1 | 8 | |g volume:31 |g year:2015 |g pages:305-311 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.meegid.2015.02.017 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25724091 |
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