Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine
The aim of this study was to determine the anti-inflammatory effects of preventive administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin or with flax-seed oil in the gut of rats, which developed chronic inflammation following administration of the pro-carcinogen N,N-dimethylhydrazine (DMH). After 28weeks administration of probiotic/prebiotic-containing diet, rats were killed and their colons were examined by immunohistological criteria, whereas cytokines were determined in the jejunal mucosa. Application of DMH triggered the production of pro-inflammatory cytokines IL-2, IL-6, IL-17, and TNF-α, expression of pro-inflammatory mediators NF-κB, COX-2 and iNOS and caused depletion of goblet cells. Supplementing the diet with L. plantarum and its combination with the prebiotic abolished DMH-induced inflammatory process in the jejunal mucosa by inhibiting the production of pro-inflammatory cytokines and by stimulation of anti-inflammatory IL-10 cytokine synthesis, whereas concentration of TGF-β1 was not influenced significantly. Diet prevented a decrease in goblet cell numbers but numbers of mast cells were lowered only moderately. However, combined treatment of rats with L. plantarum and flax-seed oil had no significant effect on the parameters examined, except for decreased expression of NF-κB, in comparison with the negative control. Results indicate that the preventive administration of probiotic L. plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin to rats with DMH-induced chronic inflammation can reduce inflammatory process in the jejunal and colon mucosa, probably indirectly, and involves down-regulation of synthesis of pro-inflammatory cytokines and suppression of NF-κB activity in mucosal cells..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
International immunopharmacology - 24(2015), 2, Seite 361-368 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Štofilová, Jana [VerfasserIn] |
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| Links: |
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| doi: |
10.1016/j.intimp.2014.12.022 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine |
| 264 | 1 | |c 2015 | |
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| 520 | |a The aim of this study was to determine the anti-inflammatory effects of preventive administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin or with flax-seed oil in the gut of rats, which developed chronic inflammation following administration of the pro-carcinogen N,N-dimethylhydrazine (DMH). After 28weeks administration of probiotic/prebiotic-containing diet, rats were killed and their colons were examined by immunohistological criteria, whereas cytokines were determined in the jejunal mucosa. Application of DMH triggered the production of pro-inflammatory cytokines IL-2, IL-6, IL-17, and TNF-α, expression of pro-inflammatory mediators NF-κB, COX-2 and iNOS and caused depletion of goblet cells. Supplementing the diet with L. plantarum and its combination with the prebiotic abolished DMH-induced inflammatory process in the jejunal mucosa by inhibiting the production of pro-inflammatory cytokines and by stimulation of anti-inflammatory IL-10 cytokine synthesis, whereas concentration of TGF-β1 was not influenced significantly. Diet prevented a decrease in goblet cell numbers but numbers of mast cells were lowered only moderately. However, combined treatment of rats with L. plantarum and flax-seed oil had no significant effect on the parameters examined, except for decreased expression of NF-κB, in comparison with the negative control. Results indicate that the preventive administration of probiotic L. plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin to rats with DMH-induced chronic inflammation can reduce inflammatory process in the jejunal and colon mucosa, probably indirectly, and involves down-regulation of synthesis of pro-inflammatory cytokines and suppression of NF-κB activity in mucosal cells. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 Elsevier B.V. All rights reserved. | ||
| 650 | 4 | |a Jejunum - drug effects | |
| 650 | 4 | |a Intestinal Diseases - metabolism | |
| 650 | 4 | |a Colon - drug effects | |
| 650 | 4 | |a Colon - metabolism | |
| 650 | 4 | |a Cytokines - metabolism | |
| 650 | 4 | |a Nitric Oxide Synthase Type II - metabolism | |
| 650 | 4 | |a Inflammation - chemically induced | |
| 650 | 4 | |a Intestinal Mucosa - pathology | |
| 650 | 4 | |a Inflammation - pathology | |
| 650 | 4 | |a Inflammation - metabolism | |
| 650 | 4 | |a Colon - pathology | |
| 650 | 4 | |a Intestinal Mucosa - metabolism | |
| 650 | 4 | |a Intestinal Diseases - drug therapy | |
| 650 | 4 | |a Inulin - pharmacology | |
| 650 | 4 | |a Intestinal Diseases - pathology | |
| 650 | 4 | |a Probiotics - therapeutic use | |
| 650 | 4 | |a Jejunum - metabolism | |
| 650 | 4 | |a Inulin - therapeutic use | |
| 650 | 4 | |a Probiotics - pharmacology | |
| 650 | 4 | |a Intestinal Mucosa - drug effects | |
| 650 | 4 | |a Cyclooxygenase 2 - metabolism | |
| 650 | 4 | |a Anti-Inflammatory Agents - therapeutic use | |
| 650 | 4 | |a NF-kappa B - metabolism | |
| 650 | 4 | |a Anti-Inflammatory Agents - pharmacology | |
| 650 | 4 | |a Inflammation - drug therapy | |
| 700 | 1 | |a Szabadosová, Viktória |4 oth | |
| 700 | 1 | |a Hrčková, Gabriela |4 oth | |
| 700 | 1 | |a Salaj, Rastislav |4 oth | |
| 700 | 1 | |a Bertková, Izabela |4 oth | |
| 700 | 1 | |a Hijová, Emília |4 oth | |
| 700 | 1 | |a Strojný, Ladislav |4 oth | |
| 700 | 1 | |a Bomba, Alojz |4 oth | |
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| 773 | 1 | 8 | |g volume:24 |g year:2015 |g number:2 |g pages:361-368 |
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