Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism
The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway..
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2015 |
|---|---|
| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
|---|---|
| Enthalten in: |
Chemistry & biology - 22(2015), 3, Seite 336-341 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Stavrinides, Anna [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| doi: |
10.1016/j.chembiol.2015.02.006 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
OLC1959996509 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1959996509 | ||
| 003 | DE-627 | ||
| 005 | 20230714152342.0 | ||
| 007 | tu | ||
| 008 | 160206s2015 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.chembiol.2015.02.006 |2 doi | |
| 028 | 5 | 2 | |a PQ20160617 |
| 035 | |a (DE-627)OLC1959996509 | ||
| 035 | |a (DE-599)GBVOLC1959996509 | ||
| 035 | |a (PRQ)c1857-d21e9a7e0e90e490782ca17ba51e54bdc3d5a2fecf96ab33f35bec76fea821550 | ||
| 035 | |a (KEY)0250883420150000022000300336unlockingthediversityofalkaloidsincatharanthusrose | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 540 |a 570 |q DNB |
| 084 | |a BIODIV |2 fid | ||
| 100 | 1 | |a Stavrinides, Anna |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 540 | |a © 2015 The Authors 2015 | ||
| 650 | 4 | |a Brief Communication | |
| 700 | 1 | |a Tatsis, Evangelos C |4 oth | |
| 700 | 1 | |a Foureau, Emilien |4 oth | |
| 700 | 1 | |a Caputi, Lorenzo |4 oth | |
| 700 | 1 | |a Kellner, Franziska |4 oth | |
| 700 | 1 | |a Courdavault, Vincent |4 oth | |
| 700 | 1 | |a O'Connor, Sarah E |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Chemistry & biology |d Cambridge, Massachusetts : Cell Press, 1994 |g 22(2015), 3, Seite 336-341 |w (DE-627)182385094 |w (DE-600)917827-2 |w (DE-576)04561380X |x 1074-5521 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:2015 |g number:3 |g pages:336-341 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.chembiol.2015.02.006 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25772467 |
| 856 | 4 | 2 | |u http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=4372254&tool=pmcentrez&rendertype=abstract |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a FID-BIODIV | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_4116 | ||
| 951 | |a AR | ||
| 952 | |d 22 |j 2015 |e 3 |h 336-341 | ||