Genome‐guided investigation of plant natural product biosynthesis
The medicinal plant Madagascar periwinkle, C atharanthus roseus (L.) G. Don, produces hundreds of biologically active monoterpene‐derived indole alkaloid ( MIA ) metabolites and is the sole source of the potent, expensive anti‐cancer compounds vinblastine and vincristine. Access to a genome sequence would enable insights into the biochemistry, control, and evolution of genes responsible for MIA biosynthesis. However, generation of a near‐complete, scaffolded genome is prohibitive to small research communities due to the expense, time, and expertise required. In this study, we generated a genome assembly for C. roseus that provides a near‐comprehensive representation of the genic space that revealed the genomic context of key points within the MIA biosynthetic pathway including physically clustered genes, tandem gene duplication, expression sub‐functionalization, and putative neo‐functionalization. The genome sequence also facilitated high resolution co‐expression analyses that revealed three distinct clusters of co‐expression within the components of the MIA pathway. Coordinated biosynthesis of precursors and intermediates throughout the pathway appear to be a feature of vinblastine/vincristine biosynthesis. The C. roseus genome also revealed localization of enzyme‐rich genic regions and transporters near known biosynthetic enzymes, highlighting how even a draft genome sequence can empower the study of high‐value specialized metabolites. It is now possible to generate within a single research lab a plant genome sequence that provides a near complete representation of genic regions: here, we report on the genome assembly of the medicinal plant Catharanthus roseus , a producer of many specialized metabolites, including several anti‐cancer compounds. We show how the draft genome sequence can facilitate identification, discovery, and an improved understanding of the genetic repertoire involved in specialized secondary metabolism..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:82 |
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| Enthalten in: |
The plant journal - 82(2015), 4, Seite 680-692 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kellner, Franziska [VerfasserIn] |
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| Links: |
Volltext |
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| Themen: |
Alkaloid |
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| doi: |
10.1111/tpj.12827 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a The medicinal plant Madagascar periwinkle, C atharanthus roseus (L.) G. Don, produces hundreds of biologically active monoterpene‐derived indole alkaloid ( MIA ) metabolites and is the sole source of the potent, expensive anti‐cancer compounds vinblastine and vincristine. Access to a genome sequence would enable insights into the biochemistry, control, and evolution of genes responsible for MIA biosynthesis. However, generation of a near‐complete, scaffolded genome is prohibitive to small research communities due to the expense, time, and expertise required. In this study, we generated a genome assembly for C. roseus that provides a near‐comprehensive representation of the genic space that revealed the genomic context of key points within the MIA biosynthetic pathway including physically clustered genes, tandem gene duplication, expression sub‐functionalization, and putative neo‐functionalization. The genome sequence also facilitated high resolution co‐expression analyses that revealed three distinct clusters of co‐expression within the components of the MIA pathway. Coordinated biosynthesis of precursors and intermediates throughout the pathway appear to be a feature of vinblastine/vincristine biosynthesis. The C. roseus genome also revealed localization of enzyme‐rich genic regions and transporters near known biosynthetic enzymes, highlighting how even a draft genome sequence can empower the study of high‐value specialized metabolites. It is now possible to generate within a single research lab a plant genome sequence that provides a near complete representation of genic regions: here, we report on the genome assembly of the medicinal plant Catharanthus roseus , a producer of many specialized metabolites, including several anti‐cancer compounds. We show how the draft genome sequence can facilitate identification, discovery, and an improved understanding of the genetic repertoire involved in specialized secondary metabolism. | ||
| 540 | |a Nutzungsrecht: 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd | ||
| 540 | |a 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd. | ||
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| 700 | 1 | |a Cepela, Jason |4 oth | |
| 700 | 1 | |a Habermann, Marc |4 oth | |
| 700 | 1 | |a Steuernagel, Burkhard |4 oth | |
| 700 | 1 | |a Clissold, Leah |4 oth | |
| 700 | 1 | |a McLay, Kirsten |4 oth | |
| 700 | 1 | |a Buell, Carol Robin |4 oth | |
| 700 | 1 | |a O'Connor, Sarah E |4 oth | |
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